不同培养条件对黄栀子愈伤的生长和绿原酸类物质积累的影响

本文以生长指数、绿原酸类物质含量和每升培养基中绿原酸的产量为评价标准,研究了培养基种类、碳源、蔗糖浓度、光照条件及水解酪蛋白(CH)浓度对黄栀子愈伤组织的生长及其次级代谢产物绿原酸类物质积累的影响,旨在为生产绿原酸类物质提供依据。研究显示,培养条件对黄栀子愈伤组织生长状况和绿原酸类物质积累有显著影响,适合生长的培养条件与适合绿原酸类物质积累的条件并不一致;适合黄栀子愈伤组织生长的最佳培养条件为:以MS为基础培养基,麦芽糖+蔗糖为碳源、糖浓度为3%、半光照条件下鲜重增长指数最大,达到47.11%。适合绿原酸类物质积累使得总产量达到最大的最佳培养条件为:MS培养基、蔗糖为碳源、蔗糖浓度为5%、不添加水解酪蛋白、半光照条件下培养,最高产量达每升培养基含35.55 mg绿原酸类物质。

Effects of Different Culture Conditions on Callus Growth and Chlorogenic Acid Accumulation in Gardenia jasminoides Ellis

Using the growth index, the content of chlorogenic acids, and the production of chlorogenic acid per liter of medium as evaluation criteria, the effects of different culture media, carbon sources, sucrose concentrations, illumination conditions, and casein hydrolysate (CH) concentrations on callus growth and the accumulation of the secondary metabolite chlorogenic acids in Gardenia jasminoides Ellis were studied. The results indicated that different culture conditions had significant effects on the growth of Gardenia jasminoides Ellis and the accumulation of chlorogenic acids, and conditions suitable for growth were not optimal for the accumulation of chlorogenic acids. The optimal conditions for growth were as follows: Murashige and Skoog (MS) medium was used as basal medium, sucrose and maltose were used as carbon sources (at a concentration of 3%), and an alternating period of light and dark was applied. Under the above conditions, the highest fresh weight gain index was obtained (47.11%). The optimal conditions for the accumulation of chlorogenic acids were as follows: MS medium, 5% sucrose as the carbon source, no added CH, and application of an alternating period of light and dark. Under the above conditions, the highest yield of chlorogenic acids was 35.55 mg per liter of medium.