首页 | 本学科首页   官方微博 | 高级检索  
     

介孔材料SBA-15固定化胰蛋白酶的研究
引用本文:董颖超,齐涛,秦玉昌,王丽娜,李军国,张懿. 介孔材料SBA-15固定化胰蛋白酶的研究[J]. 现代食品科技, 2007, 23(11): 19-21
作者姓名:董颖超  齐涛  秦玉昌  王丽娜  李军国  张懿
作者单位:中国科学院过程工程研究所,北京,100080;中国农业科学院饲料研究所,北京,100081;中国科学院过程工程研究所,北京,100080;中国农业科学院饲料研究所,北京,100081
摘    要:在酸性条件下以正硅酸乙酯(TEOS)为硅源、非离子表面活性剂Pluronic P123为模板剂合成了介孔分子筛SBA-15,将其作为载体,对胰蛋白酶进行了固定化研究.研究了固定化条件对固定化酶量的影响,以及固定化酶的热稳定性和操作稳定性.结果表明,当酶液浓度大于5 mg/mL,固定化时间10 h时,胰蛋白酶的固定化效果最好,固定化酶量可达23.6 mg/g.固定化酶的热稳定性与游离酶相比有了显著的提高,且固定化酶具有较好的操作稳定性,连续反应6批次后,酶的剩余活性仍保持在40 %以上.

关 键 词:介孔材料  SBA-15  胰蛋白酶  固定化酶
文章编号:1673-9078(2007)11-0019-04
收稿时间:2007-08-15
修稿时间:2007-06-292007-08-15

Immobilization of Trypsin on Mesoporous Silica Material
DONG Ying-chao,QI Tao,QIN Yu-chang,WANG Li-n,LI Jun-guo,ZHANG Yi. Immobilization of Trypsin on Mesoporous Silica Material[J]. Modern Food Science & Technology, 2007, 23(11): 19-21
Authors:DONG Ying-chao  QI Tao  QIN Yu-chang  WANG Li-n  LI Jun-guo  ZHANG Yi
Affiliation:1.Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China;2.Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 10008, China
Abstract:The hexagonal silica mesoporous material SBA-1 5 with ordered 2-dimensional channel structure was used as a support to immobilize the enzyme trypsin which was assembled in the channel of SBA-15 by immersion method. The effects of immobilization conditions such as enzyme concentration, pH value and time on the absorbed amount of enzyme in SBA-15, the thermal and operational stabilities of the immobilized enzyme were studied. And the best enzyme concentration and immobilization time were 5 mg/ml and 10 h, respectively, under which the absorbed amount of enzyme reached 23.6 mg/g. It was also found that the thermal and operational stabilities of the immobilized enzyme were higher than those of the free enzyme. Silica mesoporous material SBA-5 was shown to be a good support for immobilization of enzyme.
Keywords:silica mesoporous material   SBA- 15   trypsin   immobilized enzyme
本文献已被 CNKI 维普 万方数据 等数据库收录!
点击此处可从《现代食品科技》浏览原始摘要信息
点击此处可从《现代食品科技》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号