烟草种子附生细菌群落结构与多样性分析

为揭示烟草种子附生细菌的群落结构和多样性，采用分离培养法分析了6个烟草品种（烤烟K326、云烟85、云烟87及红花大金元，雪茄烟301和白肋烟L8）种子可培养附生细菌种类，并采用Illumina高通量测序技术研究了4个品种（K326、云烟85、雪茄烟301和白肋烟L8）种子附生细菌的群落结构与多样性。结果表明:6个品种种子上共分离鉴定出细菌35株，可培养优势附生细菌有假单胞菌（Pseudomonas）、芽胞杆菌（Bacillus）、肠杆菌（Enterobacte）、泛菌（Pantoea）、鞘氨醇单胞菌（Sphingomonas）等菌属。高通量测序结果表明:K326、白肋烟L8和云烟85种子的附生细菌结构上存在共性与差异，共有的优势菌有假单胞菌、泛菌、鞘氨醇杆菌（Sphingobacterium）、肠杆菌、糖芽胞杆菌（Saccharibacillus）、寡养单胞菌（Stenotrophomonas）、根瘤菌（Rhizobium）等菌属，烟草品种附生细菌多样性排序依次为云烟85＞K326＞L8＞301。 

Community structure and diversity analyses of adnascent bacteria on tobacco seeds

In order to study the community structure and diversity of adnascent bacteria on tobacco seeds, an isolated culture method was used to analyze the cultivable adnascent bacteria on tobacco seeds of six cultivars (flue-cured tobacco K326, Yunyan 85, Yunyan 87 and Honghuadajingyuan, cigar tobacco 301, and burley tobacco L8). Illumina high-throughput sequencing technology was adopted to investigate the community structure and diversity of the adnascent bacteria on the seeds of K326, Yunyan 85, 301, L8. The results showed that a total of 35 bacterial strains were isolated from the seeds of the six tobacco cultivars, and the dominant cultivable adnascent bacteria were Pseudomonas, Bacillus, Enterobacter, Pantoea, Sphingomonas, etc. High-throughput sequencing results indicated that there were similarities and differences in the structure of adnascent bacterial communities of K326, L8 and Yunyan 85 seeds, and the shared dominant bacteria genera were Pseudomonas, Pantoea, Sphingobacterium, Enterobacter, Saccharibacillus, Stenotrophomonas, Rhizobium, etc. The cultivars in the order of seed adnascent bacteria diversity were Yunyan 85 > K326 > L8 > 301.