| 烟草中游离植物甾醇的GC/MS/SIM分析 |
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| 引用本文： | 黄龙,王进元,罗诚浩,宋旭艳,马舒翼,柯炜昌.烟草中游离植物甾醇的GC/MS/SIM分析[J].烟草科技,2006(10):41-45. |
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| 作者姓名： | 黄龙 王进元 罗诚浩 宋旭艳 马舒翼 柯炜昌 |
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| 作者单位： | 武汉烟草(集团)有限公司技术中心,430051 |
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| 摘 要： | 建立了一种采用二氯甲烷提取、微孔滤膜过滤、气相色谱/质谱/选择离子监测(GC/MS/SIM)定量测定烟草中游离植物甾醇的方法,并采用该法测定了部分烟叶样品、霉变烟叶和部分卷烟样品中的游离植物甾醇含量。结果表明:①该法的检测限为5.12~8.43ng/g,回收率在93%~103%之间,RSD<6%;②香料烟的麦角甾醇含量最高,其次是烤烟,白肋烟最低;③卷烟烟丝中的游离植物甾醇含量与其烟气中苯并a]芘的释放量呈显著正相关。结论:该法适合批量烟叶样品中甾醇的定量分析。
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| 关 键 词： | 气相色谱/质谱/选择离子监测 烟草 植物甾醇 霉变 苯并[a]芘 |
| 文章编号： | 1002-0861(2006)10-0041-05 |
| 修稿时间： | 2006年5月15日 |
Determination of Free Phytosterols in Tobacco by Gas Chromatography/Mass Spectroscopy/Selected Ion Monitoring |
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| HUANG LONG,WANG JIN-YUAN,LUO CHENG-HAO,SONG XU-YAN,MA SHU-YI,KE WEI-CHANG.Determination of Free Phytosterols in Tobacco by Gas Chromatography/Mass Spectroscopy/Selected Ion Monitoring[J].Tobacco Science & Technology,2006(10):41-45. |
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| Authors: | HUANG LONG WANG JIN-YUAN LUO CHENG-HAO SONG XU-YAN MA SHU-YI KE WEI-CHANG |
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| Abstract: | A method for determination of free phytosterols in tobacco was developed with dichloromethane extraction,micropore membrane filtration and gas chromatography/mass spectroscopy/selected ion monitor.The free phytosterols in tobacco samples,mouldy tobacco,and cigarette samples were tested.The results showed that: 1) the recovery of free phytosterols was from 93% to 103%,the detection limit was 5.12 to 8.43ng/g and RSD below 6%;2)the oriental tobacco samples had the highest ergosterol level,followed by Virginia tobacco,and burley tobacco the lowest;3) the free phytosterols in cigarette filler significantly correlated with the benzoa]pyrene delivery in mainstream cigarette smoke.The conclusion was that the method was suitable to quantitative analysis of free phytosterols in batches of tobacco samples. |
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| Keywords: | Gas chromatography/mass spectroscopy/selected ion monitoring Tobacco Phytosterols Moulding Benzo[a]pyrene |
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