GC-MS/MS法分析不同规格卷烟主流烟气醛酮类成分释放量

为考察细支、中支和常规卷烟主流烟气中醛酮类成分的释放特征，建立了同时测定卷烟主流烟气95种醛酮类香味成分的气相色谱-串联质谱（GC-MS/MS）法，采用相同烟丝配方卷制细支、中支和常规规格卷烟，检测主流烟气8种低分子量醛酮类刺激性成分和95种醛酮类香味成分。通过单因素方差分析（ANOVA）筛选出差异化合物。结果表明:①95种醛酮类香味成分标准工作曲线线性关系良好（R2>0.99），日内相对标准偏差（RSD）为1.4%~11.1%，日间RSD在0.7%~24.3%之间（其中70个化合物的RSD＜10%）；3个添加水平下，92种化合物的回收率在70%~120%之间。②共筛选出68种差异化合物，以单位焦油释放量计，常规卷烟主流烟气中有7种小分子醛酮类刺激性成分释放量（除甲醛外）均高于中支和细支卷烟。③对于沸点≤133 ℃的醛酮类香味成分，如2-己酮、乙醛和2, 3-戊二酮等，常规卷烟释放量高于中支和细支卷烟；对于沸点≥215 ℃的醛酮类香味成分（3-甲基苯乙酮和肉桂醛除外），如甲基环戊烯醇酮、香兰素和5-羟甲基糠醛等，常规卷烟烟气中的释放量显著低于中支和细支卷烟；对于沸点在133~215 ℃之间的醛酮类香味成分（2-庚酮、2-甲基四氢呋喃-3-酮和6-甲基-3，5-庚二烯-2-酮除外），如2-环戊烯-1-酮、2-甲基-2-环戊烯-1-酮和糠醛等，中支卷烟烟气中的释放量高于细支和常规卷烟。 

Effects of cigarette size on releases of aldehydes and ketones in mainstream smoke analyzed by GC-MS/MS

In order to investigate the releases of aldehydes and ketones in mainstream smoke of cigarettes of different sizes, a gas chromatography-tandem mass spectrometry (GC-MS/MS) method was developed for simultaneous determining 95 aldehyde and ketone components in mainstream cigarette smoke. The samples of slim, medium and king-sized cigarettes were prepared with the same tobacco blend. Eight irritants with lower molecular weights and 95 aroma components of aldehydes and ketones were measured. One-way analysis of variance (ANOVA) was used to screen the different compounds. The results showed that: 1) The calibration curves of all the aroma components presented good linearity (R2>0.99). The intra- and inter-day relative standard deviations (RSDs) were in the ranges of 1.4%-11.1% and 0.7%-24.3% respectively, wherein the RSDs of 70 compounds were less than 10%. The recoveries of 92 compounds ranged from 70% to 120% at three spiked levels. 2) A total of 68 different compounds were measured. The releases per unit tar for seven irritant components (except formaldehyde) with lower molecular weights from the king-sized cigarettes were higher than those from the medium and slim cigarettes. 3) For aldehydes and ketones with boiling points ≤133 ℃, such as 2-hexanone, acetaldehyde, and 2, 3-pentandione, their releases were higher from the king-sized cigarettes than those from the medium and slim cigarettes. For aldehydes and ketones (except 3-methylacetophenone and cinnamaldehyde) with boiling points ≥215 ℃, such as 2-hydroxy-3-methyl-2-cyclopentene-1-one, vanillin and 5-hydroxymethylfurfural, their releases were significantly lower from the king-sized cigarettes than those from the medium and slime cigarettes. For aldehydes and ketones (except 2-heptanone, 2-methyloxolan-3-one and 6-methyl-3, 5-heptadien-2-one) with boiling points ranged from 133 to 215 ℃, such as 2-cyclopentene-1-one, 2-methyl-2- cyclopentene-1-one and furfural, their releases were higher from the medium cigarettes than those from the slim and king-sized cigarettes.