二氧化氯及丁香、肉桂精油对禾谷镰刀菌生长和孢子萌发的抑制研究（网络首发、推荐阅读）

通过考察菌落直径、菌丝生长及孢子萌发情况，研究二氧化氯（ClO2）水溶液、丁香精油和肉桂精油对禾谷镰刀菌的抑菌效果。结果表明，ClO2水溶液、丁香精油和肉桂精油均对禾谷镰刀菌表现出显著的抑菌效果，其中，丁香精油在低浓度（20~40 µL/L air）下抑制效果不如肉桂精油，随着浓度增加，其抑制效果显著增加且优于肉桂精油。分析抑制率回归方程得到ClO2水溶液的半抑制浓度IC50为27 mg/L、丁香精油为58 µL/L air、肉桂精油为66 µL/L air。通过麦角固醇含量测定、扫描电镜观察和傅立叶红外扫描，发现ClO2和精油对禾谷镰刀菌菌丝细胞结构均有不同程度的破坏，ClO2水溶液破坏细胞膜，导致内容物渗出，高浓度丁香精油破坏细胞结构，肉桂精油则使菌体发育不良。

Inhibitory Effect of ClO2, Clove Essential Oil and Cinnamon Essential Oil Against Mycelial Growth and Spore Germination of Fusarium graminearum(Online First, Recommended Article)

The inhibitory effects of ClO2, clove essential oil and cinnamon essential oil on Fusarium graminearum were studied by observing the colony diameter, mycelial growth and spore germination. The results showed that ClO2, clove essential oil and cinnamon essential oil had significant inhibitory effect on Fusarium graminearum, among which, clove essential oil had lower inhibitory effect than cinnamon essential at low density (20~40 µL/L air), and its inhibitory effect was improved dramatically and better than cinnamon essential with the increase of density. Through the regression equation of inhibition rate, the IC50 of ClO2, clove essential oil and cinnamon essential oil were 27 mg/L, 58 µL/L air and 66 µL/L air respectively. By means of ergosterol content determination, scanning electron microscope observation and Fourier transform infrared analysis, we found that ClO2 and essential oil destroyed cell structure in different degrees, ClO2 destroyed cell membrane, leading to the exudation of contents. High density of clove essential oil destroyed cell structure, while cinnamon essential oil resulted in maldevelopment of mycelium.