气质联用法分析鱼油胶囊中脂肪酸组成

对市售深海鱼油胶囊中的脂肪酸化合物成分进行研究。通过甲酯化反应,以正己烷为溶剂直接进样,采用气相色谱—质谱联用技术对脂肪酸甲酯成分进行分离并辅助NIST检索工具分析成分。结果表明鱼油胶囊中主要含有棕榈酸、棕榈油酸、油酸、DHA、EPA等21种脂肪酸。通过面积归一化法计算出各种脂肪酸相对含量,其中不饱和脂肪酸含量接近70%。该实验方法简便快捷,可为同类产品脂肪酸含量研究提供借鉴。

Analysis of fatty acids composition in fish oil capsules by gas chromatography mass spectrometry

The fatty acid compound ingredients in fish oil capsule bought from market were studied. Through esterification reaction, using hexane as solvent with direct injection, the fatty acid methyl ester composition was separated by gas chromatography-mass spectrometry and analyzed the composition by NIST assisted search tool. The results showed that fish oil capsule was mainly composed of 21 kinds of fatty acids, including palmitic acid, palmitoleic acid,oleic acid, DHA, EPA. The relative contents of the fatty acids were calculated by area normalization method. The content of unsaturated fatty acid was close to 70%. The method is simple and fast, which can provide reference for the similar products.