| 转基因甜菜GTSB77品系特异性实时荧光聚合酶链式反应检测方法建立 |
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| 引用本文： | 刘二龙,卢丽,吕英姿,蒋湘,李嘉琪,夏柔菲.转基因甜菜GTSB77品系特异性实时荧光聚合酶链式反应检测方法建立[J].中国食品卫生杂志,2020,32(1):49-52. |
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| 作者姓名： | 刘二龙 卢丽 吕英姿 蒋湘 李嘉琪 夏柔菲 |
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| 作者单位： | 黄埔海关技术中心,广东 广州 510730,广州海关技术中心,广东 广州 510623,黄埔海关技术中心,广东 广州 510730,黄埔海关技术中心,广东 广州 510730,黄埔海关技术中心,广东 广州 510730,黄埔海关技术中心,广东 广州 510730 |
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| 基金项目： | 广东出入境检验检疫局科技项目(2018GDK50) |
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| 摘 要： | 目的 为实现转基因甜菜GTSB77的标识管理,建立其品系特异性实时荧光聚合酶链式反应(PCR)检测方法。方法 针对GTSB77的3′端外源插入片段与甜菜基因组DNA之间的邻接区序列设计引物和探针,建立GTSB77品系特异性实时荧光PCR检测方法,并对该方法的特异性、灵敏度和重复性进行检测。结果 建立的GTSB77检测方法特异性强,定量限(LOQ)为16拷贝,扩增效率为102%,重复性测试结果相对标准偏差(RSD)介于0.21%～1.66%之间。结论 建立的实时荧光PCR方法可应用于GTSB77的鉴定检测。
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| 关 键 词： | 转基因甜菜GTSB77 实时荧光聚合酶链式反应 品系特异性 |
| 收稿时间： | 2019/12/22 0:00:00 |
Establishment of event-specific quantitative real-time polymerase chain reaction for detecting genetically modified sugarbeet GTSB77 |
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| LIU Erlong,LU Li,LYU Yingzi,JIANG Xiang,LI Jiaqi and XIA Roufei.Establishment of event-specific quantitative real-time polymerase chain reaction for detecting genetically modified sugarbeet GTSB77[J].Chinese Journal of Food Hygiene,2020,32(1):49-52. |
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| Authors: | LIU Erlong LU Li LYU Yingzi JIANG Xiang LI Jiaqi and XIA Roufei |
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| Affiliation: | Huangpu Customs Technology Center, Guangdong Guangzhou 510730, China,Guangzhou Customs Technology Center, Guangdong Guangzhou 510623,China,Huangpu Customs Technology Center, Guangdong Guangzhou 510730, China,Huangpu Customs Technology Center, Guangdong Guangzhou 510730, China,Huangpu Customs Technology Center, Guangdong Guangzhou 510730, China and Huangpu Customs Technology Center, Guangdong Guangzhou 510730, China |
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| Abstract: | Objective For implementation of labeling regulations, an event-specific real-time polymerase chain reaction (PCR) method for quantitative detection of sugarbeet GTSB77 was established. Methods The specific primer pairs and probe based on the 3''flanking sequence of GTSB77 were designed. The specificity, sensitivity and repeatability of the developed method were examined, respectively. Results The specificity test showed it specific for GTSB77 detection. The limit of quantification(LOQ)was 16 copies and the amplification efficiency was 102%. Reapeatability of the established method was assessed and the relative standard deviation(RSD) ranged from 0.21%-1.66%. Conclusion This event-specific real-time PCR method is suitable for identification of GTSB77. |
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| Keywords: | Sugarbeet GTSB77 real-time polymerase chain reaction event-specific |
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