一起雪卡毒素中毒事件中裸胸鳝毒性的研究

目的研究厦门一起引发食物中毒的裸胸鳝毒性及种类,以预防雪卡毒素中毒。方法对中毒事件进行流行病学调查,采集裸胸鳝样品共5份,采用小鼠生物法、雪卡毒素免疫膜检测试剂盒检测毒性大小,高效液相色谱-串联质谱法测定太平洋雪卡毒素(P-CTX-1),同时提取中毒鱼肉组织中的mtDNA,PCR技术扩增细胞色素b(Ctyb)后测序鉴定中毒鱼种。结果小鼠生物法试验检测5份样品,鱼肉毒性为无毒性(ND)～0.052 MU/g,内脏毒性为ND～0.23 MU/g;高效液相色谱-串联质谱法未检出P-CTX-1。5种鱼种经鉴定均为裸胸鳝属(Gymnothorax),其中中毒鱼种经基因测序为波纹裸胸鳝(Gymnothorax undulates)。结论引发该起食物中毒的是一种含有雪卡毒素的波纹裸胸鳝。

Study of the toxicity of the Gymnothorax related to a ciguatera fish poisoning case

Objective To study the toxicity and species of the Gymnothorax caused the ciguatera fish poisoning in Xiamen. Methods Epidemiological investigation was conducted and five kind of Gymnothorax were collected from the market and determined by mouse bioassay, Cigua-Check kit and high performance liquid chromatograph y-tandem mass spectrometry (HPLC-MS/MS). The mtDNA were extracted from Gymnothorax, and Ctyb gene segment were amplified using PCR techniques and sequenced. Results The result turned out to be not detected (ND)-0.052 MU/g in muscle, and ND-0.23 MU/g in viscera by mouse bioassay among five species. P-CTX-1 was not detected by HPLC-MS/MS. Five species of Gymnothorax were identified, and the toxic Gymnothorax implicated in food poisoning was Gymnothorax undulates. Conclusion This food poisoning was caused by ciguatoxin-carrying Gymnothorax and the species was determined to be Gymnothorax undulates based on sequence analysis.