蛋白质芯片快速诊断铁缺乏的准确性分析

对蛋白质芯片检测平台快速诊断铁缺乏人群的准确性与发现铁储量不足的能力进行分析。方法 采用免疫比浊法与蛋白质芯片法进行血清铁蛋白与可溶性转铁蛋白受体的方法学对照试验。计算蛋白质芯片法的灵敏度、特异度、阳性似然比、阴性似然比、约登指数及Kappa值。对两种方法所得铁储量进行配对比较t检验。结果 蛋白质芯片法诊断铁缺乏与比浊法相比,灵敏度较高、特异性较好、具有较高的诊断价值与真实性；其判断铁缺乏的能力与比浊法具有高度的一致性。但蛋白质芯片法筛查出更多有可能铁储量不足的情况,能更好地起到快速筛查的作用。结论 蛋白质芯片法作为快速筛查工具能同时检测SF和sTfR,对铁缺乏的诊断准确性较高,可用于进一步的应用研究。

Accuracy analysis of orotein microarray in iron deficiency rapid diagnosis

To analyze the ability and accuracy of protein microarray for body iron stores (BIS) and iron deficiency diagnosis.MethodsThe protein microarray was compared with commercially available traditional tests for serum ferritin and soluble transferrin receptor, evaluated by indicator including the sensitivity, specificity, Youden''s index, likelihood ratio, Kappa index. The BIS results were compared between protein microarray and immunoturbidimetry by paired t test. ResultsThe protein microarry showed higher sensitivity, better specifcity and coherence with immunoturbidimetry on iron deficiency estimates. At the same time, more BIS insufficient were discoverd by protein microarray.ConclusionThe protein microarray, a new rapid diagnosis technique, could be used to simultaneously detect SF and sTfR, opposed high diagnosis potencial on iron deficiencyestimates and it was valuable for more application research.