循环伏安法快速测定饮料及果冻中柠檬黄和日落黄

以玻碳电极为工作电极、铂柱电极为对电极、饱和甘汞电极为参比电极的三电极体系,利用电化学循环伏安法对5种市售商品中的柠檬黄和日落黄含量进行测定,并对测试方法进行优化。结果表明,在0.1~0.8μg/mL浓度范围内,循环伏安曲线中的峰电流与组分浓度间呈较好的线性关系,5种样品中柠檬黄含量为4.88~6.06 mg/kg,日落黄含量为4.37~5.22 mg/kg,均低于国家限量标准。该方法对柠檬黄和日落黄的检出限分别为1.12,1.43 mg/kg,柠檬黄与日落黄测试结果的相对标准偏差分别为2.1%,2.3%(n=6),当加标浓度为2.0 mg/kg时,目标组分的加标回收率为91.5%~114.5%。

A quick determination of the content of tartrazine and sunset yellow FCF in beverages and jelly by cyclic voltammetry

By using a three-electrode setup consisting of a working electrode made up of glassy carbon,a counter electrode made up of platinum,and a reference electrode made up of saturated calomel,the cyclic voltammetry was utilized to measure the content of tartrazine and sunset yellow FCF in five goods sold in the market and the measurement method was optimized.The result indicated by the cyclic voltammetry curve that the peak current was linearly correlated to the analyte concentration within the range of 0.1~0.8μg/mL.The concentration of tartrazine and sunset yellow FCF in the five samples were measured to be respectively in the range of 4.88~6.06 mg/kg and 4.37~5.22 mg/kg,which were both lower than the national standards.The Method Detection Limit(MDL)of this measurement of the concentration of tartrazine and sunset yellow FCF was 1.12 mg/kg and 1.43 mg/kg respectively.Their relative standard deviations of the test results for lemon yellow and sunset yellow were 2.1% and 2.3%(n=6).With satisfying accuracy,this approach featureed a recovery rate of the target component ranging from 91.5% and 114.5% when the fortified level was 2.0 mg/kg.