| 二次柱层析制备高纯度表没食子儿茶素没食子酸酯(EGCG)的工艺研究 |
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| 引用本文： | 朱斌,陈晓光.二次柱层析制备高纯度表没食子儿茶素没食子酸酯(EGCG)的工艺研究[J].食品与机械,2009,25(4). |
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| 作者姓名： | 朱斌 陈晓光 |
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| 作者单位： | 四川职业技术学院,四川,遂宁,629000 |
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| 基金项目： | 四川省教育厅自然科学科研基金项目 |
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| 摘 要： | 以商品荼多酚为原料,先用聚酰胺柱层析预分离表没食子儿茶素没食子酸酯(EGCG),再用硅胶柱层析制备高纯度的EC-CG.采用此工艺条件,利用较低等级的商品茶多酚(70%TP)可以制备出纯度达98%以上的EGCG.
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| 关 键 词： | 表没食子儿茶素没食子酸酯 聚酰胺 硅胶 茶多酚 |
Study on preparation process of high purity of epigallocatechin gallate(EGOG) by column chromatography |
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| ZHU Bin,CHEN Xiao-guang.Study on preparation process of high purity of epigallocatechin gallate(EGOG) by column chromatography[J].Food and Machinery,2009,25(4). |
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| Authors: | ZHU Bin CHEN Xiao-guang |
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| Abstract: | A method is described for purification of epigallocatechin gallate (EGCG) by employing polyamide/silica gel column chromatography. The raw material was commercial tea polyphenols. The operating conditions of the chromatographic steps were investigated, first preliminary separation of EGCG by employing polyamide column chromatography, and then preparation of high purity of EGCG by employing silica gel column chromatography. The purification of EGCG is over 98% by using relative low level tea polyphenols (70% TP) for raw materials in this process. |
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| Keywords: | Epigallocateehin gallate(EGCG) Polyamide Silica gel Tea polyphenols |
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