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| XDA-1大孔树脂对芹菜黄酮分离纯化的研究 | |
| 引用本文： | 王克勤,刘仲华,罗军武,陈静萍,陈亮.XDA-1大孔树脂对芹菜黄酮分离纯化的研究[J].食品与机械,2007,23(3):65-69. |
| 作者姓名： | 王克勤 刘仲华 罗军武 陈静萍 陈亮 |
| 作者单位： | 1. 湖南农业大学天然产物研究中心,湖南,长沙,410128;湖南省原子能农业应用研究所,湖南,长沙,410125 2. 湖南农业大学天然产物研究中心,湖南,长沙,410128 3. 湖南省原子能农业应用研究所,湖南,长沙,410125 |
| 摘 要： | 通过比较6种大孔吸附树脂对芹菜提取物静态吸附性能,筛选出大孔吸附树脂XDA-1,对它的动态吸附分离条件的上样液浓度、洗脱溶剂及洗脱速率进行研究.结果表明：XDA-1大孔吸附树脂对芹菜黄酮的静态吸附率为88.18%,解吸率98.43%.本试验优化条件为：芹菜提取物总黄酮浓度为0.7025 mg/mL,上样流速为2 BV/h,洗脱液采用2 BV/h的洗脱流速,洗脱液为4 BV/h 70%的乙醇,树脂富集倍数为8.416. |
| 关 键 词： | 大孔吸附树脂 芹菜 黄酮 吸附 |
| 修稿时间： | 2007-01-11 |
Study on separating and purification of flavonoid from Apium graveolensL.with xda-1 macroporous adsording resin |
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| WANG Ke-qin,LIU Zhong-hua,LUO Jun-wu,CHEN Jin-ping,CHEN Liang.Study on separating and purification of flavonoid from Apium graveolensL.with xda-1 macroporous adsording resin[J].Food and Machinery,2007,23(3):65-69. | |
| Authors: | WANG Ke-qin LIU Zhong-hua LUO Jun-wu CHEN Jin-ping CHEN Liang |
| Affiliation: | 1. Natural Products Research Center, Hunan Agricultural University, Changsha , Hunan 410128, China; 2. Hunan Institute of Atomic Energy Application in Agriculture, Changsha, Hunan 410125, China |
| Abstract: | By comparing static adsorption properties of flavonoid from the Celery extraction on macroporous adsording resin including AB-8, HP20, H-3520, D101, XDA-1, NKA-9, XDA-1 shows a higher adsorption capacity. The dynamic adsorption separating condition, example, sample concentration, wash solvent and purge velocity was studied. The results indicated that static state adsorption rate for flavonoid from Celery extraction is 88.8% , purge adsorption rate is 98.4%. This examination was optimized including 0. 702 5 mg/mL flavone concentration of celery extraction, 2 BV/h adding sample flow rate, 2 BV/h wash rate, 4 BV/h 70% Etethanol. XDA-1 macroporous adsording resin enrichment amount are 8. 418 multiple. |
| Keywords: | Macroporous adsording resin Celery Flavonoids Adsorption |
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