牛奶中阿莫西林含量表面增强拉曼光谱检测方法的建立

以香水百合花瓣绿色合成的银溶胶为表面增强拉曼散射(SERS)活性基底,建立了牛奶中阿莫西林含量的SERS检测方法。对SERS检测条件进行了优化,考察了银溶胶浓度、银溶胶与阿莫西林溶液的体积比和氯化钠溶液的添加与否对SERS检测的影响。试验结果表明:当银溶胶浓度为106.04μg/mL、银溶胶与阿莫西林溶液的体积比为4:1且不添加氯化钠的条件下,获得的SERS信号最强。在该条件下,阿莫西林标准溶液的浓度与1 055cm^-1处拉曼特征峰的SERS信号强度在1～1 000μg/mL范围内有良好的线性关系,方法的最低检出浓度可达到1μg/mL。3份加标牛奶中阿莫西林的加标回收率为84.51%～93.62%,相对标准偏差(RSD)为5.16%。该方法简单方便,可实现牛奶中一定浓度阿莫西林的检测。

Establishment of amoxicillin content detection method in milk by Surface Enhanced Raman Spectroscopy

In this study,the silver colloid solution biosynthesized by Lilium casa blanca petals powders was used as the Surface-enhanced Raman Scattering(SERS)active substrate to establish a SERS detection method for amoxicillin content in milk.The effect of the concentration of silver colloid solution,the volume ratio of silver colloid solution and amoxicillin solution,and the addition of sodium chloride on SERS detection were investigated to optimize the detection conditions.The results showed that,under the conditions of the concentration of silver colloid solution of 106.04μg/mL,with the volume ratio of silver colloid solution and amoxicillin solution of 4:1,and without adding sodium chloride,the SERS signal was the strongest.Under these conditions,the concentrations of amoxicillin solution had a good linear relationship with the SERS intensity at 1 055 cm-1 in the range of 1~1 000μg/mL,with a minimum detectable concentration of 1μg/mL.The recoveries of amoxicillin in the three spiked milks ranged from 84.51% to 93.62% with a relative standard deviation(RSD)of 5.16%.In conclusion,using this simple and convenient method,a certain concentration of amoxicillin in milk could be detected.