高效液相色谱法同时测定柚子中的4种黄酮苷和柠檬苦素

为同时测定柚子中芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷和柠檬苦素,研究了高效液相色谱的洗脱条件、测定波长等对测定结果的影响。结果表明,优选的测定条件为色谱柱:Bio-Bond 5μm C₁₈(4.6 mm×250 mm);进样量10μL;黄酮苷与柠檬苦素的检测波长分别为283 nm和220 nm;流动相:乙腈(A)和水(B),梯度洗脱:0 min,22%A;8 min,22%A;10 min,95%A;16 min,95%A;18 min,22%A;20 min,22%A,流速1 mL/min。该测定条件下,上述5种成分分离良好;其标准曲线相关系数均大于0.9997,检出限为0.69~1.72 mg/L,定量限为1.51~3.15 mg/L,重复性相对标准偏差小于2%。加样回收率均大于98%,加样回收率相对标准偏差均小于2%。

Simultaneous determination of four flavonoid glycosides and limonin in pomelo by HPLC

In order to simultaneously determine narirutin,naringin,hesperidin,neohesperidin and limonin in pomelo,the effects of elution conditions and measurement wavelength of high-pressure liquid chromatography on the determination results were studied.The results showed that the optimal measurement conditions were as follows.Chromatographic column of Bio-Bond 5μm C₁₈(4.6 mm×250 mm) was used,and the injection volume was 10μL;the detection wavelengths of flavonoid glycosides and limonin were 283 nm and 220 nm,respectively;The phase:included acetonitrile(A)and water(B)with gradient elution of 0 min,22%A,8 min,22%A,10 min,95%A,16 min,95%A,18 min,22%A,and 20 min,22%A,with 1 mL/min flow rate.Under the control o these conditions,the above five components were well separated;the correlation coefficient of the standard curve was greater than 0.9997,and the detection limit was 0.69~1.72 mg/L.The quantification limit was 1.51~3.15 mg/L,and the relative standard deviation of repeatability was less than 2%.The sample recovery rates were all greater than 98%,and the relative standard deviation of the sample recovery rates were all less than 2%.