| 食品中常见病原微生物的DNA条形码识别技术 |
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| 引用本文： | 钟文涛,徐越,王淑好,王芳妹.食品中常见病原微生物的DNA条形码识别技术[J].食品与机械,2021,37(2):47-51. |
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| 作者姓名： | 钟文涛 徐越 王淑好 王芳妹 |
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| 作者单位： | 国家农副产品质量监督检验中心;湖南省产商品质量监督检验研究院 |
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| 基金项目： | 湖南省市场监督管理局科技计划项目(编号:2019KJJH03)。 |
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| 摘 要： | 为探究DNA条形码技术在微生物识别方面的应用前景,对食品中常见的8种病原微生物的16S rRNA序列进行了比对,设计通用引物后,进行PCR扩增、测序及数据库检索,尝试建立一套基于DNA条形码的微生物识别方案。结果表明,扩增的V3~V6可变区域能准确区分变形杆菌纲(肠杆菌目、弧菌目、假单胞菌目)的致病菌和相关干扰菌,对芽孢杆菌纲(芽孢杆菌目)可鉴定到“属”水平。
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| 关 键 词： | DNA条形码 16S rRNA 病原微生物 识别 |
| 收稿时间： | 2020/9/15 0:00:00 |
Study on DNA barcoding identification technology of common pathogenic microorganisms in food |
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| ZHONGWentao,XUYue,WANGShuhao,WANGFangmei.Study on DNA barcoding identification technology of common pathogenic microorganisms in food[J].Food and Machinery,2021,37(2):47-51. |
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| Authors: | ZHONGWentao XUYue WANGShuhao WANGFangmei |
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| Affiliation: | (National Quality Supervision and Inspection Centre for Agricultural and Sideline Product,Changsha,Hunan 410007,China;Hunan Testing Institute of Product and Commodity Supervision,Changsha,Hunan 410007,China) |
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| Abstract: | In order to explore the application prospects of DNA barcode technology in microbial identification,the 16S rRNA sequences of 8 common pathogenic microorganisms in food were compared.The PCR amplification,sequencing and database search were conducted followed by universal primers designing,which were purposed to establish a microbial identification method based on DNA barcode.The results showed that the variable region sequence of V3~V6 amplified provided sufficient resolution for Gammaproteobacteria Enterobacteriales,including Vibrionales,and Pseudomonadales to accurately distinguish pathogenic bacteria and related interfering bacteria,corresponding to Bacillibacteria(Bacillales)could be identified to the“genus”level. |
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| Keywords: | DNA barcoding 16S rRNA pathogeny microbiology identification |
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