基于二氧化锰纳米片和核酸外切酶I构建荧光适配体传感器检测氯霉素

为创建食品中氯霉素的新型快速检测方法,以二氧化锰纳米片淬灭适配体的荧光,核酸外切酶I酶切放大荧光信号,构建了检测氯霉素的适配体传感器。结果表明:在适配体浓度50nmol/L,二氧化锰质量浓度0.05mg/mL,核酸外切酶用量0.4U/μL,酶切时间50min的最佳荧光检测条件下,线性范围为0.1~80.0nmol/L,检出限为0.08nmol/L。构建的检测方法具有操作简单,检测灵敏度高的优点,并实现了在食品样品中的准确检测。

Fluorescence aptasensor detection of chloramphenicol based on MnO2 nanosheet and Exo-I

In order to develop rapid detection method for chloramphenicol in food,in this study,a novel chloramphenicol aptasensor was constructed based on fluorescence quenching ability of MnO2 nanosheet and fluorescence amplification ability of Exo-Ⅰdigestion.Results showed that under optimal fluorescence detection conditions of 50 nmol/L concentration of aptamer,0.05 mg/mL concentration of MnO2 nanosheet,0.4 U/μL concentration of Exo-Ⅰand Exo-Ⅰdigestion time of 50 minutes,and the fluorescence intensity had a good linear relationship with the concentration of chloramphenicol(R2=0.995).The linear range of detection was 0.1~80.0 nmol/L with the detection limit of 0.08 nmol/L.The constructed detection approach had the advantages of simple operation and high sensitivity,and could realize accurate detection of chloramphenicol in food samples.