| AB-8大孔树脂分离纯化北五味子藤茎总木脂素的研究 |
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| 引用本文： | 薛雪,孟宪军,李斌,李元甦.AB-8大孔树脂分离纯化北五味子藤茎总木脂素的研究[J].食品与机械,2011,27(1). |
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| 作者姓名： | 薛雪 孟宪军 李斌 李元甦 |
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| 作者单位： | 沈阳农业大学食品学院,辽宁,沈阳,110161;辽宁省农产品深加工重点实验室,辽宁,沈阳,110161 |
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| 基金项目： | “十一五”国家科技支撑计划项目(编号:2006BAI09B04) |
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| 摘 要： | 基于紫外分光光度法测定北五味子藤茎总木脂素含量,通过计算6种大孔树脂的吸附率和解析率,筛选出分离纯化总木脂素的最佳树脂为AB-8大孔树脂。研究各工艺因素对吸附和解析效果的影响,得到分离纯化总木脂素的最佳工艺为:总木脂素浓度为1.511 mg/mL时,最大上样量约为6倍柱床体积,洗脱流速1 mL/min,蒸馏水洗脱体积为8倍柱床体积,洗脱剂95%乙醇用量为5倍柱床体积。在此工艺条件下的洗脱率达到78.89%。
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| 关 键 词： | 北五味子 藤茎 木脂素 大孔树脂 |
Study on separation and purification of total lignans from caculis of Schisandra Chinensis(Turcz.)Baill by AB-8 macroporous resin |
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| XUE Xue,MENG Xian-jun,LI Bin,LI Yuan-su.Study on separation and purification of total lignans from caculis of Schisandra Chinensis(Turcz.)Baill by AB-8 macroporous resin[J].Food and Machinery,2011,27(1). |
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| Authors: | XUE Xue MENG Xian-jun LI Bin LI Yuan-su |
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| Affiliation: | XUE Xue1,2 MENG Xian-jun1,2 LI Bin1,2 LI Yuan-su1,2(1.College of Food,Shenyang Agriculture University,Shenyang,Liaoning 110161,China,2.Provincia Key Laboratory of Further Processing of Food,China) |
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| Abstract: | According to the UV spectrophotometry method for measuring the total lignans content from caulis of Schisandra chinensis,the author screens out the best resin for obtaining pure lignans by calculating the adsorption and desorption rate of six types of macro porous resins.With the evaluation of the dosage of loading buffer,velocity of adsorption,the volume of distilled water and alcohol in the eluent,the writer then comes out with the best purification process for obtaining total lignans content from AB-8 ma... |
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| Keywords: | elution rate resin lignans purification industrial world |
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