高压脉冲电场提取枸杞多糖工艺

采用高压脉冲电场破壁方法,对影响枸杞多糖提取率(Y)的pH值(X1)、电场强度(X2)、脉冲频率(X3)、温度(X4)、液料比(X5)5个主要因素进行单因素和多因素分析。用SAS 9.1统计软件对星点设计方案的结果进行完全二次响应曲面的回归模型的拟合与模型的岭嵴分析。结果表明:当X1=8.98,X2=20.49kV/cm,X3=10520Hz,X4=61.76℃,X5=9.43:1(mL/g)时,枸杞多糖提取率最高(Ymax),为13.26%。在此基础上通过效应面法预测得出工业提取的工艺范围:X1=8.50～9.00,X2=15.00～25.00kV/cm,X3=10000～11000Hz,X4=20～40℃,X5=9:1～10:1(mL/g)。

Extraction of Polysaccharides from Chinese Wolfberry(Lycium barbarum L.) Fruits by High Intensity Pulsed Electric Fields

One-factor-at-a-time and central composite designs was used to analyze five crucial variables that influence extraction rate of polysaccharides from Chinese wolfberry fruits by high intensity pulsed electric fields(HPEF) including pH(X1),pulse number(X2),electric field strength(X3),temperature(X4) and material-to-liquid ratio(X5).Based on the experimental data of star point designs,a full quadratic response surface regression model was constructed with SAS 9.1 software and used for ridge analysis of response surfaces.When X1,X2,X3,X4 and X5 were 8.98,20.49 kV/cm,10520,61.76 ℃ and 1:9.43 g/mL,respectively,the highest polysaccharide yield,13.26%,was obtained.According to response surface predictions,the appropriate levels of X1,X2,X3,X4 and X5 for industrial extraction of polysaccharides from Chinese wolfberries were in the range of 8.50-9.00,15.00-2.00 kV/cm,10000-11000,20-40 ℃ and 1:9-1:10(g/mL),respectively.