| 波纹巴非蛤酶解过程的肽谱变化 |
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| 引用本文： | 陈忻,陈晓刚,陈杰文,孙恢礼.波纹巴非蛤酶解过程的肽谱变化[J].食品科学,2012,33(3):48-53. |
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| 作者姓名： | 陈忻 陈晓刚 陈杰文 孙恢礼 |
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| 作者单位： | 1.佛山科学技术学院理学院 2.中国科学院南海海洋研究所 3.中国科学院研究生院 |
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| 基金项目： | “十一五”国家科技支撑计划项目(2008BAD94B08);广东省2009院省合作项目(2009B091300018);广东省2010院省合作项目(2010B090900027) |
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| 摘 要： | 以波纹巴非蛤为原料，酶解1～6h制备酶解液。在SDS-PAGE电泳法测定肽谱的基础上，再利用液相色谱-质谱联用测定波纹巴非蛤酶解的肽谱变化。结果表明：酶解前3h时酶解液的肽分子的分子质量大概主要集中在100、50、2kD，酶解液大都是大分子肽或者中分子肽；酶解4、5、6h时酶解液的肽分子的分子质量基本没变化，其分子质量主要集中在400～600D，即为小分子肽，酶解4h时为酶解终点。
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| 关 键 词： | 波纹巴非蛤 活性肽 SDS-PAGE电泳法 液相色谱-质谱联用 |
Variations in Peptide Mass Fingerprints of Paphia undulata Muscle Hdyrolysates during Enzymatic Hydrolysis |
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| CHEN Xin,CHEN Xiao-gang,CHEN Jie-wen,SUN Hui-li.Variations in Peptide Mass Fingerprints of Paphia undulata Muscle Hdyrolysates during Enzymatic Hydrolysis[J].Food Science,2012,33(3):48-53. |
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| Authors: | CHEN Xin CHEN Xiao-gang CHEN Jie-wen SUN Hui-li |
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| Affiliation: | 1. College of Chemistry and Chemical Engineering, Foshan University, Foshan 528000, China；
2. South China Sea Institute of Oceanlogy, Chinese Academy of Sciences, Guangzhou 510301, China；
3. Graduate University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | Paphia undulate muscles were hydrolyzed with papain and the hydrolysates after hydrolysis for 1,2,3,4,5 h and 6 h were analyzed by SDS-PAEG and LC-MS/MS.The results revealed that the molecular mass distribution of peptides in Paphia undulate muscle hydrolysates was mostly concentrated on 100,50 kD and 2 kD during the first 3 hours of the hydrolysis,most of them large or small peptides.No substantial difference in the molecular mass distribution of peptides,which was mostly between 400 D and 600 D,was observed among the 4 h,5 h and 6 h hydrolysates,suggesting that 4 h was the end point of the hydrolysis. |
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| Keywords: | Paphia undulate bioactive peptide SDS-PAGE LC-MS |
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