新疆红景天总黄酮及多糖的分离提取及抑菌活性初探

本文用不同浓度的乙醇作为浸提剂，对药用植物红景天干药材中的总黄酮、多糖和单糖及一些苷类的混合物等成分分别进行分离提取，并运用分光光度法对总黄酮、多糖进行了含量测定。通过研究发现：50％乙醇浸泡24h左右能对总黄酮有较理想的提取（1．309％），而多糖在选用85％乙醇浸提分离其他成分后，再用水浸提时提取率较高（1．086％）。红景天的粗提物已被证实对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌、变形杆菌具有一定的抑制作用，为确定红景天中何种成分起抑菌作用，用所提取的几种成分进行抑菌实验，研究结果显示：在所选用的几种细菌中，总黄酮对金黄色葡萄球菌（抑菌圈直径：21．25mm）、大肠杆菌（抑菌圈直径：6．33mm）有抑制作用；多糖则无明显的抑菌作用。

Extraction and Bacteriostatic Activities of Chromo-cor and Saccharides from Rhodiola rosea

Extraction of the Chromo-cor and saccharides from Rhodiola rosea with different ethanol concentrations as infusions was studied in this paper. Different compounds were assayed with spectrophotometeric method. Results of the study showed that the higher amount of the Chromo-cor content (1.086%) can be extracted from Rhodiola rosea about 24 hours infusion with 50% ethanol and it is considered to be the optimum conditions for the extractions of Chromo-cor. The water extract of the residue infused by 85% ethanol is the optimum one for the extraction of saccharide from Rhodiola rosea and the calculated amount is 1. 086%.The bacteriostatic activities of the different compounds from medicinal plant Rhodiola rosea were also further studied in this experiment. The results showed: the antimicrobial activity of Chromo-cor from Rhodiola rosea on Staphylococcus aureus was the highest among the several bacteria used for the study (the diameter of the antimicrobial circle was 21.25 mm). It alsoshows weak inhibitory effect to E.coli; while the saccharide from the Rhodiola rosea does not show any antimicrobial activity to all bacteria used in this study.