Surfactin凝胶配方设计及其抑菌性能分析

为拓宽表面活性素Surfactin的应用范围,拟制备以卡波姆974p作为抑菌凝胶基质、Surfactin作为抑菌成分、三乙醇胺为中和剂的Surfactin抑菌凝胶。通过研究各成分对该抑菌凝胶的抑菌作用、pH值和黏度的影响,设计Box-Behnken响应面试验得到最佳配方,优化结果为Surfactin用量0.60%、卡波姆974p用量0.35%、三乙醇胺用量0.30%,此条件下得到的抑菌凝胶感官清亮,Surfactin性质更加稳定,有较强的瞬时杀菌活性,与溶液相比,对成熟的金黄色葡萄球菌生物膜的清除效果有显著提升,处理后的生物膜出现裂纹,颜色变浅透亮,分布稀疏,更容易被清除。

Formulation Design and Antibacterial Properties of Carbopol Loaded Surfactin Gel

A carbopol loaded-Surfactin antibacterial gel was prepared by using Surfactin as a major effective component, carbopol as a thickener and triethanolamine as a neutralizer. The formulation was optimized using response surface methodology with Box-Behnken design. Three dependent variables were considered: diameter of inhibition zone, pH and viscosity of the gel. The optimal formulation was determined as follows: Surfactin 0.60%, carbopol 0.35%, and triethanolamine 0.30%. The optimized gel was transparent and stable with effective antibacterial activity, and the disruption of mature Staphylococcus aureus biofilm by the gel was improved obviously as compared with sterile water. After being treated with the Surfactin gel, the biofilm showed lighter color and sparse distribution with a certain light transmittance and high disruption.