| AB-8型大孔吸附树脂分离纯化鼠曲草总黄酮工艺 |
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| 引用本文： | 王孟楚,李超.AB-8型大孔吸附树脂分离纯化鼠曲草总黄酮工艺[J].食品科学,2011(24). |
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| 作者姓名： | 王孟楚 李超 |
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| 作者单位： | 徐州工程学院食品工程学院; |
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| 基金项目： | 徐州工程学院培育项目(XKY2010114) |
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| 摘 要： | 采用AB-8型大孔树脂对从鼠曲草中提取的总黄酮产物进行分离纯化研究。考察各种因素对树脂吸附和洗脱效果的影响。通过实验得到最佳吸附工艺条件为上样液流速2BV/h、上样液pH4.5、上样液质量浓度1.7mg/mL;最佳洗脱工艺条件为洗脱液体积分数为60%乙醇、洗脱液流速1BV/h和洗脱液用量1.8BV。分离纯化后的总黄酮产品纯度可达35.42%。
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| 关 键 词： | 鼠曲草 总黄酮 分离纯化 |
Separation and Purification of Total Flavonoids from Gnaphlium affine D.Don Using AB-8 Macroporous Adsorption Resin |
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| WANG Meng-chu,LI Chao.Separation and Purification of Total Flavonoids from Gnaphlium affine D.Don Using AB-8 Macroporous Adsorption Resin[J].Food Science,2011(24). |
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| Authors: | WANG Meng-chu LI Chao |
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| Affiliation: | WANG Meng-chu,LI Chao*(College of Food Engineering,Xuzhou Institute of Technology,Xuzhou 221000,China) |
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| Abstract: | The separation and purification of total flavonoids from Gnaphlium affine D.Don were carried out using AB-8 macroporous adsorption resin in this study.The effects of various factors on adsorption and desorption efficiency of the resin were investigated.The optimal adsorption conditions for sample loading velocity,pH value and concentration were 2 BV/h,4.5 and 1.7 mg/mL,respectively,and the optimal desorption conditions were achieved by using 1.8 BV of 60% ethanol as desorption solvent at a flow rate of 1 BV... |
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| Keywords: | Gnaphlium affine D Don total flavonoids separation and purification |
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