高效液相色谱-串联质谱法测定香菇中23种有机磷农药

采用高效液相色谱-串联质谱法测定香菇中23 种有机磷农药残留。样品通过乙腈提取、盐析分配，得到的乙腈提取液采用C18和N-丙基乙二胺粉末分散固相萃取净化，经高效液相色谱-串联质谱仪分析，采用电喷雾正离子方式及多反应监测模式测定，基质匹配标准溶液外标法定量。方法的定量限达到0.01 mg/kg；回收率为
70.2%～105.0%，相对标准偏差为3.5%～13.0%。该方法灵敏、准确、快速，可满足香菇中多种有机磷农药残留的检测要求。

Determination of 23 Organophosphorus Pesticide Residues in Lentinus edodes by High Performance Liquid Chromatography-Tandem Mass Spectrometry

A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS-MS) method was established
for the determination of 23 organophosphorus pesticide residues in Lentinus edodes. Samples were extracted with acetonitrile
and separated by salting out method, and then the supernatants were purified using dispersive solid-phase extraction (C18 and
primary-secondary amine powder) prior to HPLC-MS-MS analysis. The analytes were identified in positive electrospray
ionization and multiple reaction-monitoring modes. The matrix-matched external standard calibration curves were used
for quantitative analysis. Under the optimal conditions, the limit of quantitation of the method was 0.01 mg/kg. The
recoveries of 23 organophosphorus pesticides in spiked real samples were 70.2%–105.0% with relative standard deviation
of 3.5%–13.0%. The method presented is sensitive, accurate and fast and can meet the requirements for the determination of
organophosphorus pesticide residues in Lentinus edodes.