广泛靶向代谢组学与转录组学联合分析胰蛋白酶对火龙果中抗氧化酶和饱和脂肪酸的调控

为了进一步研究胰蛋白酶的保鲜机制,探讨火龙果中胰蛋白酶和抗氧化酶的协同机制,根据胰蛋白酶调控火龙果的RNA-seq数据筛选活性氧(reactive oxygen species,ROS)和抗氧化酶相关基因.蛋白质-蛋白质相互作用(protein-protein interaction,PPI)网络分析结果表明,整个RO...

Widely Targeted Metabolomic and Transcriptomic Analyses Reveal the Regulation of Trypsin on Antioxidant Enzymes and Saturated Fatty Acids in Hylocereus undatus Fruit during Storage

In order to gain further insights into the mechanism of action of trypsin in fruit preservation, the synergistic mechanisms of trypsin and antioxidant enzymes were evaluated in pitaya (Hylocereus undatus). Reactive oxygen species (ROS) and antioxidant enzyme-related genes were selected based on the RNA-seq data of H. undatus regulated by trypsin. The results of protein-protein interaction (PPI) networks suggested that the entire ROS network could be a true biological scale-free network. Analysis using the Molecular Complex Detection (MCODE) plugin in Cytoscape showed that most antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidases (GPXs), were gathered in the cluster 2 of the ROS network. The differentially expressed antioxidant enzymes were further analyzed, revealing that peroxidase 5 (POD5) and CAT as the hub proteins were regulated by trypsin. In addition, all 22 saturated fatty acids identified based on targeted metabolomic data were down-regulated. Seven of the saturated lysophosphatidylethanolamine (LysoPC) and lysophosphatidylcholine (LysoPE) were the major metabolites. To sum up, trypsin can regulate the antioxidant system and inhibit the levels of saturated fatty acids to preserve pitaya. This finding may provide a new strategy for fruit preservation.