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| 制备抗氧化棉籽肽用蛋白酶的筛选 | |
| 引用本文： | 高海燕,赵镭,尹京苑,倪晋仁.制备抗氧化棉籽肽用蛋白酶的筛选[J].食品科学,2007,28(8):297-302. |
| 作者姓名： | 高海燕 赵镭 尹京苑 倪晋仁 |
| 作者单位： | 1. 上海大学生命科学学院,上海,200444 2. 中国标准化研究院食品与农业标准化研究所,北京,100088 3. 北京大学环境工程系,水沙科学教育部重点实验室,北京,100871 |
| 摘 要： | 本实验研究了由Alcalase碱性蛋白酶、Flavourzyme风味蛋白酶、Neutrase中性蛋白酶、无锡2709碱性蛋白酶、无锡精制中性蛋白酶等蛋白酶水解棉籽蛋白的水解度及水解液抗氧化活性的大小。结果表明,Alcalase碱性蛋白酶水解能力最大,水解液清除自由基的能力也最大,实际生产中为了去除多肽的苦味物质,应先用Alcalase碱性蛋白酶水解,再用Flavourzyme风味酶作用。 |
| 关 键 词： | 棉籽肽 蛋白酶 水解度 抗氧化 |
| 文章编号： | 1002-6630（2007）08-0297-06 |
| 修稿时间： | 2007-06-10 |
Selection of Proteolytic Enzymes to Prepare Antioxidative Cottonseed Peptides |
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| GAO Hai-yan,ZHAO Lei,YIN Jing-yuan,NI Jin-ren.Selection of Proteolytic Enzymes to Prepare Antioxidative Cottonseed Peptides[J].Food Science,2007,28(8):297-302. | |
| Authors: | GAO Hai-yan ZHAO Lei YIN Jing-yuan NI Jin-ren |
| Affiliation: | 1.College of Life Sciences, Shanghai University, Shanghai 200444, China ; 2.Key Laboratory of Water and Sediment Sciences, Ministry of Education, Dep~ment of Environmental Engineering, Peking University, Beijing 100871, China 3.Food and Agricultural Standardization Institute of China Standardization Institute, Beijing 100088, China |
| Abstract: | Several proteolytic enzymes(such as Alcalase, Neutrase, Flavourzyme, etc) were studied. The results showed that Alcalase had best hydrolyze ability and anti-oxidation in all enzymes. Double-enzyme of alcalase and flavourzyme can not increase the DH, but can remove the bitter of peptide. In actually production, Flavourzyme should be used by step to hydrolyze cottonseed protein after reacted with alcalase for removing the bitter of peptide. |
| Keywords: | cottonseed peptide proteases degree of hydrolysis anti-oxidation |
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