基于UPLC-QTOF-MS/MS技术分析荷叶乙酸乙酯相中主要化学成分

采用超高效液相色谱-四极杆飞行时间串质谱技术分析荷叶乙酸乙酯相中的主要化学成分,根据色谱峰的精确分子质量、母离子、碎片离子、质谱裂解规律等信息,结合荷叶中已经鉴定的化合物以及相关参考文献对化合物的结构进行鉴定。从荷叶乙酸乙酯相中共鉴定出40种化合物,包括27种黄酮类化合物、6种酚酸、4种有机酸、2种脂肪酸和1种氨基酸。黄酮类化合物是荷叶乙酸乙酯相中的主要化学成分,主要为槲皮素糖苷、山柰酚糖苷、异鼠李素糖苷和黄酮-3-O-醇类,其次为酚酸。本研究可为荷叶资源在食品加工和功能性食品开发领域的应用提供理论基础。

Characterization of Chemical Constituents in Ethyl Acetate Fraction of Lotus Leaves by Ultra-high Performance Liquid Chromatography-Quadrupole Time-of-Flight Tandem Mass Spectrometry

This research was designed to identify the major chemical constituents of the ethyl acetate fraction of lotus leaf extract by using ultra-high performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (UPLC-QTOF-MS/MS). The compounds were identified based on the accurate molecular mass, parent ions, MS/MS fragment ions, and MS/MS fragmentation pattern of each peak, along with the information about the compounds previously identified in lotus leaves as well as available literature data. Totally, 40 compounds were identified, including 27 flavonoids, 6 phenolic acids, 4 organic acids, 2 fatty acids and 1 amino acid. Flavonoids were the major chemical constituents with quercetin glycosides, kaempferol glycosides, isorhamnetin glycosides and flavan-3-ols being the predominant ones, followed by phenolic acids. This research can provide a theoretical foundation for the application of lotus leaves in the food processing industry and for health-promoting food development.