平菇多糖对四氯化碳诱导雄性昆明种小鼠肝损伤的保护作用

采用热水浸提法提取平菇多糖,研究平菇多糖对四氯化碳(CCl_4)诱导的雄性昆明种小鼠肝损伤的保护作用。将48只雄性昆明种小鼠随机分为空白对照组、模型组、阳性对照组、平菇多糖低、中、高剂量组,各组小鼠按剂量连续灌胃相应药物或平菇多糖28 d,在第22、23天,除空白对照组外其余各组小鼠通过腹腔注射CCl_4诱导肝损伤。在第29天处死小鼠,称体质量,采集血液并取出肝脏。采用试剂盒测定小鼠血清中谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartateaminotransferase,AST)、碱性磷酸酶(alkalinephosphatase,ALP)活力以及总胆固醇(totalcholesterol,TC)、甘油三酯(triglyceride,TG)、低密度脂蛋白胆固醇(low-density lipoprotein cholesterol,LDL-C)、高密度脂蛋白胆固醇(high-density lipoprotein cholesterol,HDL-C)浓度,并计算肝脏指数。制备肝组织匀浆,采用试剂盒测定肝组织中丙二醛(malondialdehyde,MDA)浓度、总抗氧化能力(total antioxidant capacity,T-AOC)以及总超氧化物歧化酶(total superoxide dismutase,T-SOD)、过氧化氢酶(catalase,CAT)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力。研究结果表明:与模型组相比,平菇多糖各剂量组小鼠体质量降低程度减缓,肝脏肿胀程度降低;小鼠血清ALT、AST、ALP活力极显著降低(P0.01),血脂水平(TC、TG、LDL-C浓度)显著下降(P0.05,P0.01),HDL-C浓度显著提高(P0.05,P0.01);小鼠肝组织中MDA浓度显著下降,T-AOC及T-SOD、CAT、GSH-Px活力显著提高(P0.05,P0.01)。综上,平菇多糖对CCl_4诱导的雄性昆明种小鼠肝损伤具有明显的保护作用。

Hepatoprotective Effect of Pleurotus ostreatus Polysaccharides on Carbon Tetrachloride-Induced Liver Injury in Male Kunming Mice

In this experiment, the hepatoprotective effect of Pleurotus ostreatus polysaccharide (POP), prepared by hot water extraction, on CCl4-induced liver injury in mice was studied. Totally 48 male Kunming mice were randomly divided into 6 groups including blank control, model, positive control, and low-, medium- and high-dose POP (POP-L, POP-M and POP-H) groups. All treatments were administered orally for 28 consecutive days. On the 22nd and 23rd day, liver injury in all mice except those in the blank control group was induced by intraperitoneal injection of CCl4. On the 29th day, body mass was measured and all mice were sacrificed to harvest blood and liver tissue. The activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP), and the concentrations of total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) in serum were measured by commercial kits and then the liver index was calculated. Liver tissue homogenate was prepared to measure the content of malondialdehyde (MDA), total antioxidant capacity (T-AOC) and the activities of total superoxide dismutase (T-SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) using commercial kits. The results showed that compared with the model group, POP treatment at each dose attenuated body mass loss and the swelling degree of liver, decreased significantly serum ALT, AST and ALP activities (P < 0.01) as well as TC, TG and LDL-C concentrations (P < 0.05, P < 0.01), and increased significantly HDL-C concentration (P < 0.05, P < 0.01). Moreover, it significantly decreased MDA level and increased T-SOD, T-AOC, CAT and GSH-Px in liver homogenate (P < 0.05, P < 0.01). In conclusion, POP has significant protective effects against CCl4-induced liver injury in male Kunming mice.