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泡沫分离法纯化枸杞多糖及其动力学过程分析
引用本文:陈亮,张炜,陈元涛,李轩领,刘海彬,高中超.泡沫分离法纯化枸杞多糖及其动力学过程分析[J].食品科学,2015,36(8):29-36.
作者姓名:陈亮  张炜  陈元涛  李轩领  刘海彬  高中超
作者单位:青海师范大学化学系,青海 西宁 810008
基金项目:科技部科技人员服务企业项目(2009GJG20039)
摘    要:采用泡沫分离法纯化枸杞多糖(Lycium barbarum polysaccharides,LBPs)。通过苯酚-硫酸法测定LBPs含量,以富集比(E)和回收率(R)为响应值,采用响应面法Box-Behnken设计,建立了三因素三水平拟合模型,优化了稀释倍数、初始pH值和气体流速对分离效果的综合影响;在验证得到的最佳工艺条件下,对枸杞多糖泡沫分离的动力学模型进行了分析。稀释倍数7.6、初始pH 3.42、气体流速295 mL/min时,富集比和回收率最高,分别为2.57和57.31%,与验证值(2.41和61.28%)的相对误差分别为6.6%和6.5%;动力学分析显示,泡沫分离枸杞多糖的过程可拟合为一级化学反应,等效速率常数为0.006 02 min-1。实验建立了一种简单、高效的枸杞多糖纯化思路,并对生产工艺过程提供了一定的理论支持。

关 键 词:枸杞多糖  泡沫分离  苯酚-硫酸法  响应面法  动力学分析  

Purification of Lycium barbarum Polysaccharides by Foam Separation and Kinetic Analysis of the Process
CHEN Liang;ZHANG Wei;CHEN Yuantao;LI Xuanling;LIU Haibin;GAO Zhongchao.Purification of Lycium barbarum Polysaccharides by Foam Separation and Kinetic Analysis of the Process[J].Food Science,2015,36(8):29-36.
Authors:CHEN Liang;ZHANG Wei;CHEN Yuantao;LI Xuanling;LIU Haibin;GAO Zhongchao
Affiliation:Department of Chemistry, Qinghai Normal University, Xining 810008, China
Abstract:Foam separation was used for purifying polysaccharides from Lycium barbarum L. (LBPs) in this paper. LBPs
were detected by phenol-sulphuric acid method and evaluated by enrichment ratio (E) and recovery (R). The effects of
dilution fold, initial pH and gas velocity on separation efficiency were assessed based on a fitting model proposed by
response surface methodology with Box-Behnken design. The optimal levels of the three factors were established and
validated. A further study was performed to kinetically investigate the purification process. As a result, when dilution
fold was 7.6, initial pH 3.42, and gas velocity 295 mL/min, maximum predicted values of E and R (2.57 and 57.31%,
respectively) were obtained, indicating a relative error of 6.6% and 6.5% compared with the experimental values (2.41 and
61.28%), respectively. Kinetic experiments showed that the process could be described as a first-order reaction with an
equivalent rate constant of 0.006 02 min-1. This study has provided a novel method for purification of LBPs, and the kinetic
process may provide valuable guidance for enhancing the purification efficiency.
Keywords:Lycium barbarum polysaccharides  foam separation  phenol-sulphuric acid method  response surface methodology  kinetic analysis  
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