毛细管电泳法手性拆分橙皮苷对映体

通过考察电泳缓冲液pH、环糊精种类和浓度、有机溶剂浓度及操作电压和温度对分离的影响,建立了一种简便快捷的高效毛细管电泳拆分橙皮苷对映体的方法。实验结果表明,以12mmol/L的羟丙基-β-环糊精为手性选择剂,200mmol/L的硼酸/甲醇(V:V=7:3,pH9.7)为电泳缓冲溶液,23kV的分离电压,在30℃下可使橙皮苷对映体达到基线分离,分离度为1.50。分析方法快速简单。

Chiral Separation of Hesperidin by Capillary Electrophoresis

A simple convenient and rapid method of capillary electrophoresis for the separation and analysis of the chiral hesperidin enantiomers was established. The effects of different chiral selectors and their concentration, buffer pH and its concentration, methanol concentration, and voltage and temperature on the separation of chiral hesperidin enantiomers were studied. The optimized conditions were: 12mmol/L of hydroxypropyl-β-cyclodextrin, 200mmol/L of borate-methanol solution in ratio of 7:3 and pH9.7 at separation voltage 23kV and temperature 30℃. The baseline separation of hesperidin enantiomers is achieved with a resolution factor of 1.50.