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光合细菌ZY2159菌株发酵生产类胡萝卜素研究
引用本文:赵峰梅,赵春贵,赵邑,赵生旺,杨素萍. 光合细菌ZY2159菌株发酵生产类胡萝卜素研究[J]. 食品科学, 2007, 28(6): 209-212
作者姓名:赵峰梅  赵春贵  赵邑  赵生旺  杨素萍
作者单位:山西省生物研究所; 山西大学生命科学与技术学院; 山西大学生命科学与技术学院 山西太原030006; 山西太原030006;
基金项目:国家自然科学基金;山西省自然科学基金;山西省科技攻关项目
摘    要:本研究探讨了发酵培养条件对红杆菌类胡萝卜素突变株ZY2159菌株细胞生长与类胡萝卜素生物合成的影响。结果表明,在温度30℃,光照2000lux,接种量10%,70%装样量,Fe3+为15mg/L条件下,在5L发酵罐培养ZY2159菌株6d可以获得最大生物量和最高类胡萝卜素生物合成量,菌体得率为15.4g/L,类胡萝卜素产量达50.9mg/L。HPLC色素分析显示,该菌株产β-胡萝卜素,占类胡萝卜素总量的22.3%。

关 键 词:光合细菌   发酵   类胡萝卜素   HPLC  
文章编号:1002-6630(2007)06-0209-04
修稿时间:2006-07-13

Fermentation Study of Carotenoids from Photosysnthetic Bacteria Strain ZY2159
ZHAO Feng-mei,ZHAO Chun-gui,ZHAO Yi,ZHAO Sheng-wang,YANG Su-ping. Fermentation Study of Carotenoids from Photosysnthetic Bacteria Strain ZY2159[J]. Food Science, 2007, 28(6): 209-212
Authors:ZHAO Feng-mei  ZHAO Chun-gui  ZHAO Yi  ZHAO Sheng-wang  YANG Su-ping
Affiliation:1.Biology Research Institute of Shanxi, Taiyuan 030006, China; 2.School of Life Science and Technology, Shanxi University, Taiyuan 030006, China
Abstract:The effects of fermentation parameters and incubation condition on cell growth and carotenoids biosynthesis in photosynthetic bacteria strain ZY2159 were investigated. The results showed that the maximal biomass yield 15.4 g/L and carotenoids yield 50.9 mg/L are obtained with cells grown at 30 ℃ and 2000 lux light intensity in 5 L fermentor for 6 days. The optimal concentration of Fe3+ for cell growth and carotenoids biosynthesis is 15 mg/L.The optimal volume of inoculation and medium for cell growth and carotenoid accumulation are 10% and 70%, respectively. β-carotenone relatively content in total carotenoids is 22.3% by HPLC.
Keywords:HPLC
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