蓝莓果汁及不同纯度蓝莓花色苷对原发性高血压大鼠血压的影响

以蓝莓果汁及不同纯度的蓝莓花色苷提取物为原料灌胃12 周龄原发性高血压大鼠（spontaneouslyhypertensive rats，SHR）和Wistar大鼠，研究花色苷提取纯化对其降压效果影响。采用蓝莓果汁（A组）（花色苷的质量分数为1.00%）及不同纯度的蓝莓花色苷提取物（花色苷的质量分数分别为8.66%（B组）、38.49%（C组）、94.42%（D组））灌胃SHR，灌胃剂量以花色苷含量计。其中，A组的灌胃剂量为94.4、472.1、944.2 mg/kg（以体质量计，下同），B组的灌胃剂量为10.9、54.5、109.0 mg/kg，C组的灌胃剂量为2.5、12.3、24.5 mg/kg，D组的灌胃剂量为1.0、5.0、10.0 mg/kg。结果表明：同剂量（花色苷当量）的蓝莓果汁与蓝莓皮渣提取物（不同纯度蓝莓花色苷提取物）相比，其对SHR的降压效果有显著差异（P＜0.05），而同剂量不同纯度的蓝莓皮渣提取物对SHR的降压效果无显著差异（P＞0.05）。其中，高剂量果汁（A组）和不同纯度蓝莓花色苷提取物（B、C、D组）灌胃SHR，灌胃后4或5 h其收缩压分别降低了38.45、32.57、33.09、33.41 mmHg。因此，蓝莓对高血压改善作用的功能成分是花色苷；蓝莓果汁的其他成分对花色苷的降血压功能有增强作用。

Effect of Blueberry Juice and Blueberry Anthocyanins with Different Purities on Blood Pressure of Spontaneously Hypertensive (SHR) Rats

This study was performed to investigate the effect of blueberry juice and blueberry anthocyanins with various purities
on blood pressure of spontaneously hypertensive (SHR) rats aged 12 weeks. The rats were separated into four groups: group A
was administered by gavage with blueberry juice (containing 1.00% anthocyanins), and groups B, C and D were administered
orally with 8.66%, 38.49% and 94.42% anthocyanins, respectively. The doses, calculated based on anthocyanins content, were
94.4, 472.1 and 944.2 mg/kg body weight (bw) for group A, 10.9, 54.5 and 109.0 mg/kg bw for group B, 2.5, 12.3 and 24.5 mg/kg bw
for group C, and 1.0, 5.0 and 10.0 mg/kg bw for group D. The results showed blueberry juice and blueberry skin extracts
containing different amounts of anthocyanins, when used at the same doses (anthocyanins equivalent), presented a significant
difference (P ＜ 0.05) in their antihypertensive effect on SHR rats. However, no significant difference in antihypertensive
activity was observed between blueberry anthocyanins with different purities when used at same doses (P ＞ 0.05). At 4 or 5
h after high-dose administration of blueberry juice and blueberry anthocyanins with three different purities, the systolic blood
pressure of SHR rats was reduced by 38.45, 32.57, 33.09, and 33.41 mmHg, respectively. Therefore, blueberry anthocyanins
can ameliorate in SHR rats, and this effect is enhanced by other active components of blueberry juice.