| 香薷多糖的提取与纯化研究 |
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| 引用本文： | 杨 超,李景恩,邱增辉,聂少平,殷军艺,李 昌,谢明勇.香薷多糖的提取与纯化研究[J].食品科学,2009,30(18):99-101. |
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| 作者姓名： | 杨 超 李景恩 邱增辉 聂少平 殷军艺 李 昌 谢明勇 |
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| 作者单位： | 南昌大学 食品科学与技术国家重点实验室 |
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| 基金项目： | 食品科学与技术国家重点实验室目标导向项目(SKLF-MB-200806)；教育部“长江学者和创新团队发展计划”项目(IRT0540)；大学生创新性实验项目资助项目(081040306) |
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| 摘 要： | 利用热水浸提、乙醇醇沉提取水溶性香薷粗多糖，脱蛋白、除色素后所得的精制多糖经DEAE-52 纤维素阴离子交换色谱得两个组分Ⅰ和Ⅱ。主要组分Ⅱ经Sephacryl S-300 葡聚糖凝胶色谱进一步纯化，得单一多糖组分，命名为HMP Ⅱ。经高效凝胶渗透色谱检测其纯度为93.55%。
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| 关 键 词： | 香薷多糖 提取 纯化 |
| 收稿时间： | 2009-04-28 |
Extraction and Purification of Polysaccharides from Herba Moslae |
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| YANG Chao,LI Jing-en,QIU Zeng-hui,NIE Shao-ping,YIN Jun-yi,LI Chang,XIE Ming-yong.Extraction and Purification of Polysaccharides from Herba Moslae[J].Food Science,2009,30(18):99-101. |
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| Authors: | YANG Chao LI Jing-en QIU Zeng-hui NIE Shao-ping YIN Jun-yi LI Chang XIE Ming-yong |
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| Affiliation: | State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China |
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| Abstract: | Crude water-soluble polysaccharides were isolated from Herba Moslae by hot water extraction and ethanol precipitation. After protein and pigment removal, the refined polysaccharides were separated by DEAE-52 cellulose anionexchange chromatography, and two fractions Ⅰand Ⅱ were obtained. The main fraction Ⅱ was further purified by Sephacryl S-300 gel chromatography and a polysaccharide named HMPⅡ was obtained. The purity of HMPⅡ was determined to be 93.55% by high performance gel permeation chromatography (HPGPC). |
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| Keywords: | polysaccharides from Herba Moslae extraction purification |
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