| 高速逆流色谱法分离制备大豆异黄酮中的大豆苷和染料木苷 |
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| 引用本文： | 江和源,台建祥,吕飞杰.高速逆流色谱法分离制备大豆异黄酮中的大豆苷和染料木苷[J].食品科学,2004,25(1):85-88. |
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| 作者姓名： | 江和源 台建祥 吕飞杰 |
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| 作者单位： | 1. 中国农业科学院茶叶研究所农业部茶叶化学工程重点开放实验室,浙江,杭州,310008
2. 中国农业科学院,北京,100081 |
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| 摘 要： | 采用高速逆流色谱法分离纯化大豆异黄酮中的大豆苦和染料木昔。溶剂系统为乙酸乙酯一醋酸一水,体积比为5:1:10,上相为固定相,下相为流动相,逆流色谱仪转速为800r/mm,流速为1.5ml/min。所得大豆昔、染料木昔经高效液相色谱分析测定,纯度分别达到98.2%和99.2%(面积归一法)。
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| 关 键 词： | 高速逆流色谱法 分离 大豆苷 染料木昔 |
| 文章编号： | 1002-6630(2004)01-0085-04 |
Separation of Daidzin and Genlstin from Soybean Isoflavones by High Speed Countercurrent Chromatography |
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| JIANG He-yuan,TAI Jian-xiang,LV Fei-jie.Separation of Daidzin and Genlstin from Soybean Isoflavones by High Speed Countercurrent Chromatography[J].Food Science,2004,25(1):85-88. |
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| Authors: | JIANG He-yuan TAI Jian-xiang LV Fei-jie |
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| Affiliation: | JIANG He-yuan1,TAI Jian-xiang2,LV Fei-jie2 |
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| Abstract: | Semlpreparation high speed coufltercurent chromatography(HSCCC)was used for the systematic optimization andpreparation of the two-phase solvent system to separate Daidzin and Genistin from soybean isoflavones extracted from soybeanseed.The two-phase solvent system composed of ethyl acetate-acetic acid-water(5:l:10 in volume ratio).The mobile phase is thelower phase nd operated at flow-rate of 1.5ml/min, while the apparatus rotated at 800r/min.High performance liquidchromatographic analysis of Daidzin and Genistin revealed that their purity was individually 98.2%and 99.2%. |
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| Keywords: | high speed countercurrent chromatography separation daidzin genistin |
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