鸡肝酯酶在有机磷和氨基甲酸酯类农药检测中的应用研究

利用农药对酯酶活力的抑制反应,分别采用游离的和固定化的鸡肝酯酶,对不同浓度的敌敌畏、敌百虫、马拉硫磷和西维因进行了测定。结果发现,在低浓度时,农药浓度的对数值与其对应的酶活抑制率之间具有较好的线性关系;并且与游离鸡肝酯酶相比,固定化酶不同程度地降低了对农药检测的下限值。敌敌畏、马拉硫磷和西维因的检测下限值依次从游离酶检测时的0．001、4．34和2．52mg/L,降低到了用固定化酶检测时的0．00004、0．0004和0．78mg/L。固定化的鸡肝酯酶对敌百虫的检测下限改善不大。

Experiment Study on Utilization of Chicken Liver Esterase in Determination of Organophosphate and Carbamate Pesticides

Based on the esterase inhibition actions of pesticides,different concentrations of dichlorvos,trichlorfon,malathion and carbaryl were determined by free and immobilized chicken liver esterase.The results showed that there are reasonable linear relationships between the logarithm of pesticides'concentrations and the inhibition ratio of esterase activity in lower concentration zones both for free and immobilized enzymes.With application of immobilized chicken liver esterase,the detection limits are 0.001,4.34 and 2.52mg/L respectively for dichlorvos,malation and carbaryl while after the free chicken liver esterase immobilized treatment,they are respectively lowered to 0.00004,0.0004 and0.78mg/L.However there is no obvious improvement in determination limit for trichlorfon with immobilized esterase.