抗消化道双歧杆菌菌株A03和A04生长特性的研究

应用改良MRS液体培养基,研究了耐消化道优良双歧杆菌菌株A03和A04的生长特性,结果表明,它们均在改良MRS培养基中生长最好,最高活菌数的lg值分别达到9.03和9.26;A03菌株以35～40℃的生长温度为佳,而A04菌株以35～37℃的生长温度为佳;它们的最适起始pH值分别为7.5和7.0,而接种量分别以2%和3%为最佳;A04比A03生长速度快,产酸能力相似,最终pH值都在3.9以下,耐酸能力均强,前者无明显的生长延滞期,10h就进入稳定期,而后者到4h后才进入对数期,12h后进入稳定期。

Study on the Growth Characteristics of Bifidobacterium A03 and A04 Toleranced to Gastrointestinal Tract

The growth characteristics of Bifidobacterium A03 and A04, which were tolerant to gastrointestinal tract, were studiedin CMRS medium. The results showed that they grew best in CMRS medium, their cell numbers were 9.03 and 9.26 log cfu/cmrespectively. Optimal temperature for their growth were 37℃, and optimal initial pH were 7.5 and 7.0 respectively. The optimalpercent of inoculability volume were 2% or 3%. A04 grew faster than A03, however, their acidic ability was similar and theywere tolerance to acid. There were not obvious delaying stage and in growth curve of A04, and it entered stability stage after 10h,but A03 entered delaying stage after 4h and entered stability stage after 12h.