高效阴离子交换色谱法同时测定菊粉酶解产物中的单糖、双糖和低聚果糖

建立了低聚果糖样品中的葡萄糖、果糖、蔗糖、蔗果三糖、蔗果四糖和蔗果五糖的高效阴离子交换色谱-脉冲安培法的定量分析方法。采用阴离子交换柱CarboaPacTM PA10（250 mm×2 mm）脉冲安培法进行检测，以氢氧化钠和醋酸钠为淋洗液进行梯度洗脱，柱温30 ℃，流速0.3 mL/min。结果表明，葡萄糖、果糖、蔗糖、蔗果三糖、蔗果四糖和蔗果五糖在质量浓度0.1～10 mg/L范围内的线性关系良好，相关系数r2大于0.996 1，各组分的相对标准偏差均在2.69%～7.21%之间。将此方法应用于菊粉酶解后反应产物的检测，结果表明该方法快速简便且灵敏度高，能满足反应样品中低聚果糖的检测要求。

Simultaneous Determination of Monosaccharides,Disaccharides, and Fructooligosaccharides in Inulin Hydrolysates by High Performance Anion Exchange Chromatography

An analytical method for the simultaneous determination of glucose, fructose, sucrose, kestose, nystose, and
fructofuranosylnystose in fructooligosaccharide products was developed by using high performance anion exchange
chromatography coupled with pulsed ampere detection (HPAEC-PAD). The analysis was performed on a CarboPacTM PA10
column（250 mm × 2 mm）by gradient elution using NaOH-NaAc as the mobile phase. The column temperature was set at 30 ℃,
and the flow rate was 0.3 mL/min. The results showed that the calibration curves developed for glucose, fructose, sucrose, kestose,
nystose and fructofuranosylnystose had a good linear relationship within the concentration range of 0.1–10 mg/L (r2 > 0.996 1).
The relative standard deviation for six repetitive determinations of each sugar was between 2.69% and 7.21%. This method is
convenient, simple, and sensitive for the detection of fructooligosaccharide products from the enzymatic hydrolysis of inulin.