响应面试验优化番木瓜籽中硫代葡萄糖苷酶解工艺

通过单因素试验和Box-Behnken试验设计响应面分析对番木瓜籽中硫代葡萄糖苷(硫苷)的酶解条件进行优化。通过气相色谱-质谱(gas chromatography-mass spectrometry,GC-MS)联用仪鉴定酶解产物中的异硫氰酸酯种类。结果表明:酶解缓冲液pH值、酶解时间对异硫氰酸酯得率有显著影响,酶解温度影响不显著;得到的最优酶解条件为酶解缓冲液pH 4.8、酶解时间40 min、酶解温度27℃,在此条件下异硫氰酸酯得率为13.5‰;GC-MS结果表明,番木瓜籽硫苷酶酶解产物中只含有异硫氰酸苄酯一种异硫氰酸酯。

Optimization of Enzymatic Hydrolysis Conditions of Glucosinolates from Carica papaya Seeds by Response Surface Methodology

The enzymatic hydrolysis of glucosinolates (GS) from Carica papaya seeds was optimized by response surface
methodology with Box-Behnken design (BBD). The resulting isothiocyanates were identified by gas chromatography-mass
spectrometry (GC-MS). The results showed that enzyme buffer solution pH and hydrolysis time had significant effects on
the yield of isothiocyanates, whereas enzymatic hydrolysis temperature had no significant effect. The optimal hydrolysis
conditions were found to be hydrolysis at 27 ℃ for 40 min in a buffer solution at pH 4.8. Under the optimized conditions,
the yield of isothiocyanates was 13.5‰. GC-MS analysis showed that only one isothiocyanate-benzyl isothiocyanate was
detected in the hydrolysate.