| 超高效合相色谱法直接检测VC方法 |
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| 引用本文： | 刘倩倩,周围,王波,王丽婷,杨盛鑫.超高效合相色谱法直接检测VC方法[J].食品科学,2014,35(16):208-211. |
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| 作者姓名： | 刘倩倩 周围 王波 王丽婷 杨盛鑫 |
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| 作者单位： | 1.甘肃农业大学食品科学与工程学院，甘肃 兰州 730070；2.甘肃出入境检验检疫局综合技术中心，;
甘肃 兰州 730000；3.西北师范大学地理与环境科学学院，甘肃 兰州 730070 |
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| 摘 要： | 建立超高效合相色谱法分离和测定VC的方法。超高效合相色谱技术集合超临界流体色谱和超高效液相色谱的技术优点，流动相以CO2为主体，甲醇（含0.05% H3PO4）为助溶剂。选用Waters CSH Fluoro-Phenyl（3.0 mm×100 mm，1.7 μm）色谱柱，流速0.6 mL/min，检测波长为245 nm，方法检出限为1.5 mg/kg，线性范围为5～200 mg/L；加标回收率范围为96.05%～101.15%；相对标准偏差为0.52%～0.76%，具有高效、检测速度快、操作简单、灵敏度高、检出限低、重复性好、实验成本低等优点。
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| 关 键 词： | 超高效合相色谱 VC 测定 食品 |
Direct Determination of Vitamin C by Ultra Performance Convergence Chromatography |
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| LIU Qian-qian,ZHOU Wei,WANG Bo,WANG Li-ting,YANG Sheng-xin.Direct Determination of Vitamin C by Ultra Performance Convergence Chromatography[J].Food Science,2014,35(16):208-211. |
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| Authors: | LIU Qian-qian ZHOU Wei WANG Bo WANG Li-ting YANG Sheng-xin |
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| Affiliation: | 1. College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China;
2. Central Laboratory of Technical Center of Gansu Entry-Exit Inspection and Quarantine Bureau, Lanzhou 730000, China;
3. College of Geography and Environment Science, Northwest Normal University, Lanzhou 730070, China |
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| Abstract: | A new method was developed for the determination of vitamin C in foods by ultra performance convergence
chromatography (UPC2). The mobile phase was a mixture of supercritical CO2 and methanol (containing 0.05% H3PO4) at a
flow rate of 0.6 mL/min. A Waters CSH Fluoro-Phenyl column (3.0 mm × 100 mm, 1.7 μm) was used, and the UV detector
was set at 245 nm. The limit of detection (LOD) was 1.5 mg/kg, and the calibration linear range was between 5 and 200 mg/L.
The spiked recoveries of vitamin C were 96.05%–101.15%, with relative standard deviation (RSD) ranging from 0.52% to
0.76%. The method is efficient, rapid, simple, sensitive, accurate, repeatable and low-cost with a low limit of detection. It
can be applied for the determination of vitamin C in foods. |
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| Keywords: | ultra performance convergence chromatography (UPC2) vitamin C determination food |
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