| 基于响应曲面法的微囊化保加利亚乳杆菌高密度培养条件优化 |
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| 引用本文： | 闫颖娟,卢俭,周剑忠,李伟,董明盛.基于响应曲面法的微囊化保加利亚乳杆菌高密度培养条件优化[J].食品科学,2014,35(17):153-159. |
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| 作者姓名： | 闫颖娟 卢俭 周剑忠 李伟 董明盛 |
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| 作者单位： | 1.忻州师范学院生物系，山西 忻州 034000；2.南京农业大学食品科技学院，江苏 南京 210095；;
3.南京卫岗乳业有限公司，江苏 南京 211100；4.江苏省农业科学院农产品加工研究所，江苏 南京 210014 |
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| 基金项目： | “十二五”国家科技支撑计划项目(2013BAD18B01-4);国家高技术研究发展计划(863计划)项目(2011AA100903);中央高校基本科研业务费专项资金项目(KYZ201419);国家农业科技成果转化资金项目(2012GB23600639);江苏高校优势学科建设工程资助项目 |
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| 摘 要： | 采用响应曲面法对微囊化保加利亚乳杆菌FMG-4高密度培养的培养基组成和培养条件进行优化,结果表明:最佳培养基配方为:在MRS培养基中添加乳清粉97.15 g/L、大豆蛋白粉10.00 g/L、酵母粉7.55 g/L、碳酸钙8.03 g/L、硫酸镁0.30 g/L、硫酸锰0.02 g/L。最优培养条件为:培养温度41.7℃、初始pH 6.9。此时保加利亚乳杆菌FMG-4细胞菌密度可达到3.18×1011 CFU/g。
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| 关 键 词： | 液芯微囊 响应曲面法 保加利亚乳杆菌 高密培养 |
Optimization of High Cell Density Culture Conditions for Microencapsulated Lactobacillus bulgaricus by Response Surface Methodology |
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| YAN Ying-juan,LU Jian,ZHOU Jian-zhong,LI Wei,DONG Ming-sheng.Optimization of High Cell Density Culture Conditions for Microencapsulated Lactobacillus bulgaricus by Response Surface Methodology[J].Food Science,2014,35(17):153-159. |
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| Authors: | YAN Ying-juan LU Jian ZHOU Jian-zhong LI Wei DONG Ming-sheng |
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| Affiliation: | 1. Department of Biology, Xinzhou Teachers University, Xinzhou 034000, China; 2. College of Food Science and Technology,;
Nanjing Agricultural University, Nanjing 210095, China; 3. Nanjing Weigang Dairy Co. Ltd., Nanjing 211100, China;
4. Institute Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China |
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| Abstract: | In this study, the medium composition and culture conditions for microencapsulated Lactobacillus bulgaricus
FMG-4 were optimized using response surface methodology. The results showed that the optimal medium for high cell
density culture of microencapsulated Lactobacillus bulgaricus FMG-4 was an MRS medium supplemented with 97.15 g/L
whey powder, 10.00 g/L soy protein powder, 7.55 g/L yeast powder, 8.03 g/L calcium carbonate, 0.30 g/L magnesium sulfate
and 0.02 g/L manganese sulfate. The optimal culture conditions were 41.7 ℃ and an initial pH of 6.9. Under the optimized
culture conditions, the density of L. bulgaricus FMG-4 reached 3.18×1011 CFU/g. |
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| Keywords: | liquid core microcapsule response surface methodology Lactobacillus bulgaricus high cell density culture |
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