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环介导等温扩增技术检测食品中酸土环脂芽孢杆菌
引用本文:周赞虎,张海艳,郑俊超,江喆敏,陈春香,何艺贞,林俊君,苏雪勤,王根芳.环介导等温扩增技术检测食品中酸土环脂芽孢杆菌[J].食品科学,2014,35(22):233-237.
作者姓名:周赞虎  张海艳  郑俊超  江喆敏  陈春香  何艺贞  林俊君  苏雪勤  王根芳
作者单位:1.厦门大学生命科学学院,滨海湿地生态系统教育部重点实验室,福建 厦门 361005;; 2.漳州出入境检验检疫局,福建 漳州 363000;3.漳州卫生职业学院,福建 漳州 363000
基金项目:福建省漳州市自然科学基金项目
摘    要:目的:利用环介导等温扩增技术建立食品中酸土环脂芽孢杆菌快速检测方法。方法:针对酸土环脂芽孢杆菌16S序列设计特异引物,再优选反应体系,用显色法检测实验结果。结果:该方法能够在63 ℃条件下1 h内检出食品中酸土环脂芽孢杆菌,所设计的引物有良好的特异性;灵敏度达6.7 CFU/mL(弱阳性)。结论:该方法具有高效、特异性强和敏感性高等特点,可满足酸土环脂芽孢杆菌快速检测筛选的要求。

关 键 词:环介导等温扩增  酸土环脂芽孢杆菌  食品  

Loop-Mediated Isothermal Amplification (LAMP) for Detection of Alicyclobacillus acidoterrestris in Foods
ZHOU Zan-hu,ZHANG Hai-yan,ZHENG Jun-chao,JIANG Zhe-min,CHEN Chun-xiang,HE Yi-zhen,LIN Jun-jun,SU Xue-qin,WANG Gen-fang,ZHENG Tian-ling.Loop-Mediated Isothermal Amplification (LAMP) for Detection of Alicyclobacillus acidoterrestris in Foods[J].Food Science,2014,35(22):233-237.
Authors:ZHOU Zan-hu  ZHANG Hai-yan  ZHENG Jun-chao  JIANG Zhe-min  CHEN Chun-xiang  HE Yi-zhen  LIN Jun-jun  SU Xue-qin  WANG Gen-fang  ZHENG Tian-ling
Affiliation:1. Key Laboratory of Coastal and Wetland Ecosystems, Ministry of Education, School of Life Sciences, Xiamen University,; Xiamen 361005, China; 2. Zhangzhou Entry-Exit Inspection and Quarantine Bureau, Zhangzhou 363000, China; 3. College of Zhangzhou Health Vocational, Zhangzhou 363000, China
Abstract:Purpose: A loop-mediated isothermal amplification (LAMP) method was established for the detection of
Alicyclobacillus acidoterrestris in foods. Methods: After optimization of the reaction conditions of LAMP including the
concentrations of primers, reaction time and amplification temperature, the LAMP method was developed, and its sensitivity
and specificity were evaluated. Results: The method was capable of rapidly and specifically detecting A. acidoterrestris in
foods within 1 hour at a constant temperature of 63 ℃. The sensitivity of the method was 6.7 CFU/mL and the specificity
was 100%. Conclusions: The LAMP method is efficient, highly sensitive and specific, and suitable for the rapid detection of
A. acidoterrestris in various food samples.
Keywords:loop-mediated isothermal amplification (LAMP)  Alicyclobacillus acidoterrestris  food
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