小麦酯酶电化学生物传感器构建及其对敌敌畏的检测

以来源于植物的小麦酯酶（plant-esterase from wheat flour，wPLaE）为检测酶源，利用纳米金（gold nanoparticles，AuNPs）和多壁碳纳米管（multi-walled carbon nanotubes，MWNT）的协同电催化放大作用，构建一种可用于有机磷农药检测的新型电化学酶传感器NF/wPLaE-CS/AuNPs/MWNT/GCE。以敌敌畏为对象，采用方波伏安法实现对其简便、低成本的检测，检出限为4 μg/L（RSN=3）。将其应用于实际样品生菜和大米中敌敌畏的检测，回收率为95.4%～107.2%，表明所制备的植物酯酶生物传感器在实际应用中具有可行性。此外，该生物传感器也具有良好的稳定性和抗干扰能力。本研究可为植物酯酶在农药检测中的应用开发提供技术支撑。

Construction of Wheat Esterase Electrochemical Biosensor and Its Application in Dichlorvos Detection

A new enzyme-based electrochemical sensor NF/wPlaE-CS/AuNPs/MWNT/GCE was constructed using wheat esterase by taking advantage of the synergistic electro-catalytic amplification effect of gold nanoparticles (AuNPs) and multi-walled carbon nanotubes (MWNT) on the electrochemical signal. The sensor provided a simple and low-cost approach for the detection of dichlorvos with square wave voltammetry, and the detection limit was 4 μg/L (RSN = 3). The biosensor was used to measure dichlorvos spiked in lettuce and rice samples with recoveries ranging from 95.4% to 107.2%, indicating the biosensor was suitable for the detection of dichlorvos pesticides in actual samples. In addition, the biosensor exhibited good stability and inference resistance. This study can provide technical support for the application of plant esterase in pesticide detection.