| 沙蒿多糖的提取、纯化工艺研究 |
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| 引用本文： | 张继,高义霞,武光朋,王云普. 沙蒿多糖的提取、纯化工艺研究[J]. 食品科学, 2007, 28(5): 125-127 |
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| 作者姓名： | 张继 高义霞 武光朋 王云普 |
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| 作者单位： | 西北师范大学生命科学学院； 西北师范大学高分子研究所 甘肃兰州730070； 甘肃兰州730070； |
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| 摘 要： | 利用传统的水煮醇沉法从沙蒿子中提取可溶性多糖,通过正交试验确定多糖的优化条件;采用Sevag+木瓜蛋白酶法脱蛋白;多糖采用琼脂糖电泳进行纯度检查;并用苯酚-硫酸法测定多糖含量。结果表明:最佳提取温度70℃,提取时间1h,料液比1:150;琼脂糖电泳表明沙蒿多糖是均一组分,纯度为96．45%。
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| 关 键 词： | 沙蒿多糖 提取 正交试验 纯化 |
| 文章编号： | 1002-6630（2007）05-0125-03 |
| 修稿时间： | 2006-06-23 |
Study on Technology of Extracting and Purifying Artemisia sphaerocephala Krasch Polysaccharides |
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| ZHANG Ji,GAO Yi-xia,WU Guang-peng,WANG Yun-pu. Study on Technology of Extracting and Purifying Artemisia sphaerocephala Krasch Polysaccharides[J]. Food Science, 2007, 28(5): 125-127 |
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| Authors: | ZHANG Ji GAO Yi-xia WU Guang-peng WANG Yun-pu |
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| Affiliation: | 1.College of Life Science,Northwest Normal University,Lanzhou 730070,China; 2.Institute of Polymers,Northwest Normal University,Lanzhou 730070,China |
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| Abstract: | A water-soluble polysaccharide was extracted from Artemisia sphaerocephala Krasch by methods of boiling water and ethanol deposition.The optimal conditions were determined by the othoganal test.The crude polysaccharide was obtained after removal of protein by Sevag and papain enzymolysis method.Moreover,purify was detected by agarose gel electrophoresis. The content of polysaccharides was measured by phenol-sulfuric acid method.The results showed that the optimal extraction conditions are:70℃,1 h,and the ratio of Artemisia seeds powder and water 1:150.Artemisia seed polysacchatides were shown to be a homogenous component by electrophoresis.In addition,the total content of Artemisia seed polysaccharide is 96.45%. |
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| Keywords: | Artemisia seed polysaccharides extraction orthogonal test purification |
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