蛭弧菌消除海产品中潜在致病性弧菌的研究

噬菌蛭弧菌具有裂解病原菌的特殊功效,可以用作生物净化因子。本实验从海洋环境中分离到4株Bh04系列蛭弧菌(Bh04-4,Bh04-41a,Bh04-A+和Bh04-1f)。以41株弧菌为蛭弧菌的宿主菌,本文研究了蛭弧菌对海产品中常见弧菌的裂解消除能力,以探索其对由弧菌所引起的食物中毒等流行性传染疾病的预防效果。实验结果表明,41株弧菌中共有36菌株可被4株蛭弧菌(Bh04-4,Bh04-41a,Bh04-A+和Bh04-1f)中的任一株所裂解,占总试验菌株的87.8%;对副溶血弧菌、非01非0139群霍乱弧菌、溶藻胶弧菌这三种常见致病弧菌的裂解率分别达88.9%、83.3%、81.8%。本研究结果揭示了蛭弧菌作为生物消除剂在由弧菌所引起的食物中毒等流行性传染疾病的防治方面有潜在应用价值。

Lysis Study of Bdellovibrios on Seafood-Borne Potentially Pathogenic Vibrios

It is a fact that Bdellovibrio can lyse gram-negative pathogenic bacteria. In this study, 4 strains of Bdellovibrio sp., designated Bh04-4, Bh04-41a, Bh04-A and Bh04-1f, were respectively isolated from seawaters using Bh04 as a host bacterium. 41 strains of vibrios from either aquatic waters or seafood were used as hosts. After confirmation of being Bdellovibrio sp. by electron microscopy and specific PCR method, lytic ability on 41 vibrios was assayed. Results showed that taking all four Bdellovibrios together, 36 lysed out of 41 strains, amounting to 87.8% lysis ability. The lysis abilities on Vibrio parahaemolyticus, Vibrio cholerae and Vibrio alginolyticus were 88.9%, 83.3%, 81.8% respectively. Results of this study fully demonstrated the potential application of Bdellovibrio on lysing vibrios from seafood origin.