| 枯草芽孢杆菌JMUKC2产肌苷摇瓶发酵培养基的优化 |
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| 引用本文： | 杨尚彤,肖安风,倪辉,杨远帆,杨哲,蔡慧农.枯草芽孢杆菌JMUKC2产肌苷摇瓶发酵培养基的优化[J].食品科学,2012(11):167-171. |
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| 作者姓名： | 杨尚彤 肖安风 倪辉 杨远帆 杨哲 蔡慧农 |
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| 作者单位： | 集美大学生物工程学院;集美大学食品微生物与酶工程研究中心;厦门市食品生物工程技术研究中心 |
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| 基金项目： | 福建省自然科学基金项目(2011J01225);集美大学中青年创新团队专项(2010A006);集美大学科研基金项目(2010N5009) |
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| 摘 要： | 利用单因素试验和正交试验对肌苷生产菌枯草芽孢杆菌JMUKC2的摇瓶发酵培养基进行优化,优化后的最佳发酵培养基配方为葡萄糖140g/L、玉米浆20g/L、酵母膏17g/L、尿素7g/L、硫酸铵20g/L、MgSO4.7H2O1g/L、KH2PO47g/L、CaCO320g/L。培养基经优化后,生物量比优化前提高了40.67%,肌苷产量比优化前提高了46.57%。
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| 关 键 词： | 肌苷 枯草芽孢杆菌 摇瓶发酵 培养基优化 |
Culture Medium Optimization for Inosine Production by Bacillus subtilis JMUKC2 in Shake Flasks |
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| YANG Shang-tong,XIAO An-feng,NI Hui,YANG Yuan-fan,YANG Zhe,CAI Hui-nong.Culture Medium Optimization for Inosine Production by Bacillus subtilis JMUKC2 in Shake Flasks[J].Food Science,2012(11):167-171. |
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| Authors: | YANG Shang-tong XIAO An-feng NI Hui YANG Yuan-fan YANG Zhe CAI Hui-nong |
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| Affiliation: | 1,2,3,(1.College of Bioengineering,Jimei University,Xiamen 361021,China; 2.Research Center of Food Microbiology and Enzyme Engineering Technology,Jimei University,Xiamen 361021,China; 3.Food Bioenginering Research Center of Xiamen,Xiamen 361021,China) |
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| Abstract: | This paper describes the optimization of the fermentation medium for inosine-overproducing Bacillus subtilis JMUKC2 in shakes flask using one-factor-at-a-time and orthogonal array design methods.The optimal fermentation medium was composed of 140 g/L glucose,20 g/L corn liquor,17 g/L yeast,7 g/L urea,20 g/L ammonium sulfate,1 g/L MgSO4·7H2O,7 g/L KH2PO4 and 20 g/L CaCO3.The resulting biomass and inosine production were increased by 40.67% and 46.57% compared with those before the optimization,respectively. |
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| Keywords: | inosine Bacillus subtilis shake flask fermentation culture medium optimization |
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