苹果皮总黄酮对D-半乳糖致衰老模型小鼠抗氧化能力的影响

目的：探究苹果皮总黄酮（flavonoids from apple pericarp，FAP）对D-半乳糖致衰小鼠的抗氧化作用。方法：采用皮下注射D-半乳糖制备衰老小鼠模型。筛选50 只健康昆明小鼠（雌雄各半）随机分为模型组、正常对照组和低、中、高剂量实验组。于造模第16天后，给低、中、高剂量组小鼠分别灌胃50、100、200 mg/kg FAP，持续45 d后，采用Morris水迷宫和小鼠避暗仪测试小鼠学习记忆能力，并采集血液、取脑、肝、心、肾组织，测定各组织丙二醛（malondialdehyde，MDA）含量、超氧化物歧化酶（superoxide dismutase，SOD）活性及谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）活力。结果：与正常对照组相比，模型组小鼠学习记忆能力减退，除心之外，脑、肝、肾、血液MDA含量显著增加（P＜0.05），SOD活性和GSH-Px活力显著下降（P＜0.05）；与模型组相比，除低、高剂量组的血液SOD活性与GSH-Px活力及低剂量组心脏GSH-Px活力无显著提高（P＞0.05），其他各组织心、脑、肝、肾SOD活性与GSH-Px活力均显著提高（P＜0.05）；但各剂量组与正常对照组相比，中剂量组各指标均无显著差异性（P＞0.05），而低、高剂量组MDA含量显著高于正常对照组（P＜0.05），SOD活性和GSH-Px活力显著低于正常对照组（P＜0.05）。结论：经口给予小鼠一定剂量的FAP有明显的抗氧化的作用，且其有剂量效应。

Effect of Flavonoids from Apple Pericarp on Antioxidant Functions in D-Galactose-Induced Aging Mice

Objective: This study aimed to examine the antioxidant effect of total flavonoids from apple pericarp (FAP)in D-galactose-induced aging mice. Methods: The aging model was established by hypodermic injection of D-galactose.Fifty healthy Kunming mice were chosen and randomly divided into five groups (n=10, half male and half female): normalcontrol, model control, low- (50 mg/kg), middle- (100 mg/kg) and high-dose (200 mg/kg) FAP treatment groups. From thesixteenth day after the injection, the mice from the three treatment groups were intragastrically administrated with FAPfor 45 successive days. The learning and memory ability of mice were tested using the darkness-avoidance and Morriswater maze methods on the forty-sixth day. Then, the mice were killed, and blood, brain, liver, heart and kidney tissueswere collected for the measurement of MDA contents, and SOD activities and GSH-Px activities. Results: Compared withthe normal control group, the learning and memory ability of the mice in the model control group were decreased; theMDA contents of brain, liver, kidney and blood except for heart tissue were increased significantly (P＜0.05), whereas theactivities of SOD and GSH-Px were decreased significantly (P＜0.05). Compared with the model control group, except forthe activities of blood SOD and GSH-Px in the mice from the high- and low-dose groups as well as heart GSH-Px activityin the mice from the low-dose group, which were not significantly enhanced (P＞0.05), both enzyme activities revealed asignificant increase in all other investigated tissues (P＜0.05). When comparing each dose group with the normal controlgroup, there were no significant differences in the various parameters for the middle-dose group, yet the low- and high-dosegroups indicated a significant increase in MDA content and a significant decrease in SOD and GSH-Px activities (P ＜ 0.05).Conclusion: Orally administered FAP improve antioxidant functions in aging mice, showing a dose-effect relationship.