| 氯化锦葵色素-3-半乳糖苷对TNF-α诱导内皮细胞炎症的抑制作用 |
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| 引用本文： | 王健,黄午阳,李春阳,郑其升,刘娅梅.氯化锦葵色素-3-半乳糖苷对TNF-α诱导内皮细胞炎症的抑制作用[J].食品科学,2014,35(9):241-245. |
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| 作者姓名： | 王健 黄午阳 李春阳 郑其升 刘娅梅 |
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| 作者单位： | 1.江苏省农业科学院农产品加工研究所，江苏 南京 210014；2.南京农业大学食品科技学院，江苏 南京 210095；;
3.江苏省农业科学院 国家兽用生物制品技术研究中心，江苏 南京 210014 |
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| 基金项目： | 国家自然科学基金青年科学基金项目(NSFC31101264);江苏省自然科学基金项目(RK2011677);江苏省农业自主创新项目(cx(12)5029) |
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| 摘 要： |
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| 关 键 词： | 氯化锦葵色素-3-半乳糖苷 人脐静脉内皮细胞 肿瘤坏死因子 炎症反应 细胞间黏附因子 核转录因子 |
Inhibitory Effect of Malvidin-3-Galactoside Chloride on TNF-α-Induced Inflammation in Endothelial Cells |
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| WANG Jian,HUANG Wu-yang,LI Chun-yang,ZHENG Qi-sheng,LIU Ya-mei.Inhibitory Effect of Malvidin-3-Galactoside Chloride on TNF-α-Induced Inflammation in Endothelial Cells[J].Food Science,2014,35(9):241-245. |
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| Authors: | WANG Jian HUANG Wu-yang LI Chun-yang ZHENG Qi-sheng LIU Ya-mei |
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| Affiliation: | 1. Institute of Farm Product Processing, Jiangsu Academy of Agricultural Science, Nanjing 210014, China; 2. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; 3. National Technical Research Centre of Veterinary Biological Products, Jiangsu Academy of Agricultural Science, Nanjing 210014, China |
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| Abstract: | Objective: The effect of malvidin-3-galactoside chloride (Mv-3-gal-Cl) on TNF-α-induced inflammation in
human umbilical vein endothelial cells (HUVEC) was investigated in this study. Methods: HUVEC were treated with TNF-α
(10 μg/L) alone and combined with Mv-3-gal-Cl (1, 10, 50 and 100 μmol/L), respectively. DMSO was used as control. The
soluble ICAM-1 protein content in cell supernatant was detected by ELISA. The change in ICAM-1 mRNA expression
level in cells was detected by RT-PCR. The expression of ICAM-1 and IкBα was assessed by Western blotting. The
activity of NF-кB was evaluated by immunofluorescence. Results: Mv-3-gal-Cl appeared to specifically down-regulate the
TNF-α-induced cell surface expression of ICAM-1 and ICAM-1 mRNA expression and protein release from HUVEC cells
in a concentration-dependent manner. The inhibitory effect of Mv-3-gal-Cl on protein expression might be associated with
the suppression of NF-κB in HUVECs. Conclusion: Mv-3-gal-Cl can inhibit TNF-α-induced inflammation in HUVECs. The
anti-inflammatory effect of Mv-3-gal-Cl provides a theoretical basis for preventing and treating pro-inflammatory cytokinesinduced
endothelial dysfunction and cardiovascular diseases resulting from inflammation. |
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| Keywords: | malvidin-3-galactoside chloride (Mv-3-gal-Cl) human umbilical vein endothelial cell (HUVEC) tumor necrosis factor-α inflammatory effect intercellular cell adhesion molecule-1 (ICAM-1) NF-kappa B |
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