虫草多糖苹果果醋的保健功能评价

目的：虫草多糖与果醋均具有对人体有益的功效，该实验将两者结合，并通过小鼠连续灌胃体内实验，考察虫草多糖果醋抗疲劳、增加免疫力、降血脂和降血糖等方面的功效。方法：首先从蛹虫草子实体中提取虫草多糖。然后以苹果为原料经过酒精发酵和醋酸发酵制备苹果果醋原液，经调配制备苹果果醋。按实验设计添加不同剂量的虫草多糖制备虫草多糖果醋。对成年小鼠进行不同剂量的虫草多糖果醋灌胃后，通过小鼠的负重游泳时间、肝糖原含量和游泳后血乳酸含量测定等判定虫草多糖果醋提高小鼠抗疲劳能力的效果。通过抑制迟发型变态反应和增强碳廓清能力测定判定虫草多糖果醋提高小鼠免疫能力的效果。对成年小鼠在饲喂高脂饲料的同时进行不同剂量的虫草多糖果醋灌胃后，通过血清总胆固醇和甘油三酯水平测定判定虫草多糖果醋对高血脂的预防效果。对成年小鼠高血糖模型进行不同剂量的虫草多糖果醋灌胃后，通过测定血糖值判定虫草多糖果醋的降血糖效果。结果：通过抗疲劳实验研究发现，虫草多糖果醋组与空白对照组相比小鼠的负重游泳时间显著提高，肝糖原含量显著升高，游泳后血乳酸含量显著降低。通过增强免疫力实验研究发现，虫草多糖果醋组与空白对照组相比，耳肿胀度明显下降，廓清指数和吞噬指数显著提高。通过辅助降血脂实验发现，虫草多糖果醋组与饲喂高脂饲料的空白对照组相比，血清总胆固醇和甘油三酯水平显著降低，小鼠体质量显著低于空白对照。通过辅助降血糖实验发现，虫草多糖果醋组与空白对照组相比，血糖水平显著降低。并且各种生物活性实验结果均与虫草多糖果醋中虫草多糖的剂量成正比。虫草多糖果醋的功效要好于虫草多糖和果醋单独使用的功效。结论：虫草多糖果醋具有缓解体力疲劳、增强免疫力、辅助降血脂和辅助降血糖的保健功能。

Healthcare Functions of Apple Cider Vinegar Fortified with Cordyceps militaris Polysaccharides

Objectives: To evaluate the anti-fatigue, immune-enhancing, auxiliary lipid-lowering and auxiliary hypoglycemiceffects of apple cider vinegar fortified with Cordyceps militaris polysaccharides orally administered to mice. Methods:Cordyceps militaris polysaccharides were extracted from the fruit bodies of Cordyceps militaris. Apple cider vinegar wasprepared by alcoholic and subsequent acetous fermentation of apple juice and formulated into a functional beverage withthe addition of the fungal polysaccharides. After adult mice were orally administered with different dosages of the applevinegar beverage, weight-loaded forced swimming time, liver glycogen content and blood lactate content after swimmingwere determined to evaluate the anti-fatigue effect of the beverage. Suppression of delayed type hypersensitivity and carbonclearance enhancement were conducted to determine the immune-enhancing function. After adult mice were fed with ahigh-fat diet and simultaneously administered orally with different dosages of the fortified beverage, serum total cholesterol(TC) and triglyceride (TG) concentrations were detected to evaluate the auxiliary lipid-lowering effect in mice. Afteradult hyperglycemic mice were orally administered with different dosages of the beverage, blood glucose concentrationwas detected to evaluate the auxiliary hypoglycemic effect in vivo. Results: In anti-fatigue tests, the weight-loaded forcedswimming time of mice was significantly increased, while liver glycogen level was significantly elevated and the areaunder the curve of blood lactate was significantly reduced in the treatment group when compared with the blank group.The swelling degree of ears was significantly reduced, and the clearance index and the phagocytosis index weresignificantly increased, as indicated by the immune-enhancing effects of the beverage. Its auxiliary lipid-loweringeffect indicated serum TC and TG levels were significantly reduced and the body weight of mice was lower than thatof the control. As for its auxiliary hypoglycemic activity, blood glucose concentration was significantly decreased.In addition, all these biological activities were dosage dependent. The efficacy of the fortified beverage was betterthan that of Cordyceps militaris polysaccharides and apple cider vinegar separately. Conclusion: Apple cider vinegarfortified with Cordyceps militaris polysaccharides has anti-fatigue, immune-enhancing, auxiliary lipid-lowering andauxiliary hypoglycemic effects in mice.