| L-苹果酸对苹果酸天冬氨酸穿梭转运蛋白及酶基因表达的作用研究 |
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| 引用本文： | 吴军林,吴清平,韦明肯,吴慧清,周小燕.L-苹果酸对苹果酸天冬氨酸穿梭转运蛋白及酶基因表达的作用研究[J].食品科学,2006,27(11):229-232. |
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| 作者姓名： | 吴军林 吴清平 韦明肯 吴慧清 周小燕 |
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| 作者单位： | 中国科学院南海海洋研究所； 广东省微生物研究所广东省菌种保藏与应用重点实验室； 广东省微生物研究所广东省菌种保藏与应用重点实验室 |
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| 基金项目： | 广州市科技攻关重大项目（2004Z-E6001）;广东省科技计划项目（2004B40101011） |
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| 摘 要： | 采用半定量RT-PCR方法,对小鼠给予L-苹果酸后肝线粒体苹果酸天冬氨酸穿梭相关的两种线粒体酶:线粒体天冬氨酸氨基转移酶(mitochondrialaspartateaminotransferase,mAST)及线粒体苹果酸脱氢酶(mitochondrialmalatedehydrogenase,mMDH)及线粒体内膜上的天冬氨酸谷氨酸转运蛋白(AGC)与α-酮戊二酸苹果酸转运蛋白(OMC)的基因表达进行检测,探讨了L-苹果酸对肝线粒体苹果酸天冬氨酸穿梭的关键酶及转运蛋白mRNA表达规律。半定量RT-PCR结果表明:剂量组小鼠肝线粒体转运蛋白AGCmRNA的表达显著高于空白对照组,而mAST、mMDH及转运蛋白OMCmRNA的表达无显著性差异。由此可知,L-苹果酸能促进TCA循环及苹果酸天冬氨酸穿梭,其分子生物学机理与L-苹果酸提高AGC的基因表达水平有关。
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| 关 键 词： | L-苹果酸 苹果酸天冬氨酸穿梭 转运蛋白 |
| 文章编号： | 1002-6630（2006）11-0229-04 |
| 收稿时间： | 2006-08-01 |
| 修稿时间： | 2006-08-01 |
Effect of L-malate on Gene Expression of Proteins and Enzymes Related to the Malate-aspartate Shuttle |
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| WU Jun-Lin,WU Qing-Ping,WEI Ming-Ken,WU Hui-Qing,ZHOU Xiao-Yan.Effect of L-malate on Gene Expression of Proteins and Enzymes Related to the Malate-aspartate Shuttle[J].Food Science,2006,27(11):229-232. |
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| Authors: | WU Jun-Lin WU Qing-Ping WEI Ming-Ken WU Hui-Qing ZHOU Xiao-Yan |
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| Affiliation: | 1.South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,China; 2.Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,Guangdong Institute of Microbiology,Guangzhou 510070,China; 3.Graduate University of the Chinese Academy of Sciences,Beijing 100049,China |
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| Abstract: | To investigate the expression change of two key enzymes in mitochondria and carried proteins (AGC and OMC) in inner member of mitochondria related to malate-aspartate shuttle,the expression of these proteins was examined in liver of mice treated L-malate by methods of RT-PCR. RT-PCR was used to analyze the expression of key enzymes (mAST and mMDH) mRNA and carried protein (AGC and OMC) mRNA after treatment of L-malate. The results indicated that the expression of AGC mRNA was increased significantly in the L-malate treated group compared with control group. There were no difference in the levels of mAST,mMDH and OMC mRNA. The results predict a potential molecule mechanism that L- malate increased capability of the malate-aspartate shuttle by enhancing the level of AGC mRNA. |
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| Keywords: | L-malate malate-aspartate shuttle carried protein |
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