| 白葡萄酒废酵母蛋白及多糖的提取工艺研究 |
| |
| 引用本文： | 屈慧鸽,于小飞,张玉香,程显好,王晓玲,杨建志.白葡萄酒废酵母蛋白及多糖的提取工艺研究[J].食品科学,2007,28(9):315-318. |
| |
| 作者姓名： | 屈慧鸽 于小飞 张玉香 程显好 王晓玲 杨建志 |
| |
| 作者单位： | 鲁东大学生命科学学院； 鲁东大学生命科学学院 山东烟台264025； 山东烟台264025； |
| |
| 摘 要： | 本实验主要研究了从白葡萄酒废酵母中提取蛋白和多糖的方法。结果表明:经处理后的酵母悬浮液在超声波功率600W,作用时间4s,间歇时间2s,作用90次的条件下,促溶效果最好。经超声波处理后,再添加0.6%(W/V)的木瓜蛋白酶,在55℃,pH5.5下,反应时间为30h,提取液中蛋白含量达到9.84g/L。提取蛋白后的残渣,用碱法提取多糖,含量达58.69%。
|
| 关 键 词： | 白葡萄酒废酵母 蛋白 多糖 超声波 木瓜蛋白酶 |
| 文章编号： | 1002-6630（2007）09-0315-04 |
| 修稿时间： | 2007-07-29 |
Study on Extraction Techniques of Protein and Polysaccharide from Waste Yeast of White Wine |
| |
| QU Hui-ge,YU Xiao-fei,ZHANG Yu-xiang,CHENG Xian-hao,WANG Xiao-ling,YANG Jian-zhi.Study on Extraction Techniques of Protein and Polysaccharide from Waste Yeast of White Wine[J].Food Science,2007,28(9):315-318. |
| |
| Authors: | QU Hui-ge YU Xiao-fei ZHANG Yu-xiang CHENG Xian-hao WANG Xiao-ling YANG Jian-zhi |
| |
| Affiliation: | College of Life Science,Ludong University,Yantai 264025,China |
| |
| Abstract: | The extracted techniques of protein and polysaccharide from white wine waste yeast were studied.The results showed:The best disaggregated effect of yeast suspension by ultrasonic is disposed in 600W,acting 4s,interval 2s,90 times.Then,put 0.6% papain into the yeast suspension which treat with ultrasonic,pH 5.5,55 ℃,reaction 28 h,the protein content of the extraction was 9.84 g/L.The polysaccharide obtained from the residue which extracted protein by NaOH solution.The polysaccharide content of extraction was 58.69%. |
| |
| Keywords: | white wine waste yeast protein polysaccharide ultrasonic papain |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《食品科学》浏览原始摘要信息 |
|
点击此处可从《食品科学》下载全文 |
|
